Carrera de Biotecnología

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34800

Browse

Filters

2022 - 202527

Settings

Has files: YesSubject: search.filters.subject.ACTIVIDAD ANTIOXIDANTE

Search Results

Now showing 1 - 10 of 27
  • No Thumbnail Available
    Item
    Evaluación de la actividad antioxidante y el tiempo de vida útil de un extracto obtenido a partir de la mora (Rubus glaucus)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Culqui Arcos, Evelyn Johanna; Fernández Rivero, Danae
    In recent years, interest innatural products has increased considerably, thanks to their therapeutic properties, especially their antioxidant activity. Among these products are extracts, which can be obtained from red fruits. However, to ensure their quality, it is crucial to determine their shelf life. An outstanding example of red fruits rich innatural antioxidants with phenolic compounds is the blackberry (Rubus glaucus). In this study, blackberry extracts were obtained using a two-factor experimental design, in which ethanol concentration and extraction time were evaluated. The phenolic content was determined using the Folin-Ciocalteu method, where the results identified as the best treatment the one that presented the highest phenolic content, reaching 62.594 milligram equivalents of gallic acid per gram of dry weight, under an ethanol concentration of 50 percent and an extraction time of 20 minutes. In addition, its antioxidant activity was evaluated using the DPPH method, obtaining an inhibition percentage of 90.544 percent. Similarly, a value of 161.003 equivalent micromoles of Trolox per gram of dry weight was recorded. Finally, the stability of the extract was analyzed for 30 days and a shelf life of 33.6540 days when stored in amber glass bottles was established using the Minitab statistical tool with a confidence level of 95 percent. Its behavior followed a first-order kinetic model, with a rate constant of 0.0031 days.
  • No Thumbnail Available
    Item
    Evaluación de la actividad antioxidante de las cremas elaboradas a base del extracto de hojas y raíces de Plantago major L y Buddleja globosa
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Chicaiza Coba, Sebastián Nicolás; Fernández Rivero, Danae
    The present study highlights the importance of evaluating the antioxidant capacity of plant extracts, specifically from matico (Buddleja globosa) and plantain (Plantago major) leaves, due to their potential application in the pharmaceutical industry and in the formulation of dermocosmetics. These extracts represent a rich source of phenolic compounds, known to combat oxidative stress, prevent premature skin aging and promote skin health. To obtain the extracts, the maceration method was used, followed by the quantification of the total phenol content (TPC), total flavonoids (TFC) and total tannins (TTC) using the Folin-Ciocalteu, aluminum chloride and Folin-Ciocalteu methods, respectively. The antioxidant capacity was determined using the DPPH method for both the extracts and a dermocosmetic cream. The results obtained showed TPC values of 15,930, 9,209, 2,400 and 2,682 mg GAE per g, while TFC values were 2,577, 2,319, 0.358 and 0.657 mg QE per g. TTC values reached 10,770, 7,944, 1,423 and 3,331 mg TAE per g, corresponding to matico and plantain leaves and roots. Regarding antioxidant capacity, the extracts presented values of 12,463 and 11,070 micromoles ET per g, while the cream showed a content of 7,694 micromoles ET per g. These results highlight the potential of matico leaf as a promising natural resource for developing dermocosmetic products with antioxidant properties, establishing a solid foundation for future research and innovative applications in this field.
  • No Thumbnail Available
    Item
    Evaluación de la capacidad antioxidante y antiinflamatoria de un extracto obtenido a partir de la seda de maíz (Zea mays)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Ulpo Galarza, Nayeli Narcisa; Fernández Rivero, Danae
    The lack of knowledge of the bioactive properties of agroindustrial residues represents a potential waste of valuable natural resources. In Ecuador, around 1.38 million tons of corn are produced annually, equivalent to millions of kilograms of silk, a by-product discarded during corn harvesting. Faced with this challenge, new alternatives have been explored for using corn silk native to the Sierra region as an antioxidant and anti-inflammatory potential. In this study, silk extracts were obtained from a three-factor experimental design, where ethanol concentration, extraction time, and solvent mass to volume ratio were evaluated. The phenolic content was determined by the Folin-Ciocalteu method and the treatment with 70 percent ethanol for 30 minutes and a mass and solvent volume ratio of 1 in 30 was established as the extract with the highest phenolic content of 69,903 milligram gallic acid equivalents per gram of dry weight. The antioxidant capacity of the best extract was evaluated by DPPH assay, where 71.185 percent inhibition was obtained. The anti-inflammatory activity was performed using the albumin denaturation assay, where the inhibition showed percentages from 14.988 to 54.787. The analyses carried out revealed that corn silk contains a significant amount of phenolic compounds with antioxidant and anti-inflammatory properties, suggesting that this agro-industrial waste could be valorized in the pharmaceutical and cosmetological industry, in order to promote the use of natural resources.
  • No Thumbnail Available
    Item
    Evaluación de la actividad antioxidante y antiinflamatoria de carotenoides microencapsulados a partir de los residuos de la naranjilla (Solanum quitoense)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Sailema Criollo, Anthony Erick; López Hernández, Orestes Darío
    The percentage of food waste has due to the fact that companies do not correctly use the entire product. Consequently, research has been carried out in order to take advantage of these waste, highlighting naranjilla for its high nutritional content, particularly carotenoids, a bioactive compound with antioxidant and anti-inflammatory properties capable of preventing various diseases. In the present study, carotenoid extraction was performed out at different plant material/solvent ratios and extraction times. A statistical analysis was then applied to optimize the two factors, obtaining a concentration of 2,287 mg per liter. Next, it was microencapsulated with a polymeric combination of gum arabic and maltodextrin. Subsequently, the microencapsulation efficiency was evaluated qualitatively by FT-IR and quantitatively with 91,74 percent efficiency. The antioxidant activity was determined using the DPPH method, achieving an inhibition percentage of 63,00 and 49,52 for the extract and the microencapsulate, respectively. Finally, the anti-inflammatory capacity was evaluated with an inhibition percentage of 56,42 for the extract and 41,42 in the microencapsulated. The results obtained indicate that the higher the concentration of carotenoids in the sample, the better results will be achieved. Therefore, the purpose of this study is to take advantage of the residues of naranjilla with a high content of carotenoids with antioxidant and anti-inflammatory properties that can be used in new products of industrial interest.
  • No Thumbnail Available
    Item
    Evaluación de la capacidad antioxidante y antiinflamatoria de polifenoles microencapsulados procedentes de los residuos de tamarindo (Tamarindus indica)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Mendoza García, Damaris Katiusca; López Hernández, Orestes Darío
    Tamarindus indica is a fruit widely used in the production of various commercial products, generating a large amount of agroindustrial waste per year. Tamarind seeds represent a source for obtaining bioactive compounds of interest such as polyphenols. The present study seeks to take advantage of these wastes by determining the optimal conditions for extraction and microencapsulation of polyphenols to evaluate their antioxidant and anti-inflammatory capacity. The influence of extraction time and solvent concentration on obtaining polyphenols was studied. The best extraction conditions were at a solvent concentration of 53 percent for a time of 80 minutes, providing a polyphenol content of 516.521 mg GAE per gram of plant material. Spray-drying microencapsulation using maltodextrin as the encapsulating agent evidenced a percentage efficiency of 87.066 percent. The efficiency of microencapsulation was also evaluated by means of infrared spectroscopy (FT-IR). The DPPH method was applied to establish the antioxidant potential of the extract and microencapsulation, reaching values of 80.085 percent and 48.916 percent, respectively. Finally, the anti-inflammatory activity was determined through the percentage of inhibition of the denaturation of egg albumin and bovine serum albumin (BSA), obtaining inhibition percentages in each of the concentrations studied. The results obtained in this research show the potential of tamarind residuals with biological activities and their possible application in different industries.
  • No Thumbnail Available
    Item
    Evaluación de la vida útil de un extracto con propiedades antioxidantes obtenido de hojas de arándano (Vaccinium corymbosum)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Aguilar Sisalema, Jennifer Victoria; Fernández Rivero, Danae
    Interest in the use of natural products has increased in recent years due to the therapeutics proprieties they can have on health, one of them being antioxidant activity. Among these products are extracts that can be made from plant waste; however, to ensure their quality, it is necessary to determine their shelf life. One of waste with antioxidants of natural origin, specifically phenolic compounds, are blueberry (Vaccinium corymbosum) leaves. Ultrasound-assisted extraction was used to obtain the extract, where the use of a 70 percent ethanol concentration, a ratio of 1 gram of plant matter to 10 milliliters of solvent and a temperature of 50 degrees Celsius made it possible to obtain a higher concentration of phenolic compounds with a value of 136,85 milligrams of gallic acid per gram of dry weight. Subsequently, its antioxidant activity was determined using DPPH method, where an inhibition of 80,78 percent was recorded, while in Trolox equivalents it was 476,93 micromoles per gram of dry weight, which is considered as a high antioxidant activity of this plant species. Finally, the shelf life on the extract when stored in amber glass jars at a refrigeration temperature ranging from 2 to 8 degrees Celsius was found to be 2,02 weeks, following first order kinetic modelling with a rate constant of 0,0413 per week.
  • No Thumbnail Available
    Item
    Evaluación de la vida útil de un extracto obtenido de la cáscara de piña (Ananas comosus) con propiedades antioxidantes y antiinflamatorias
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Nuñez Ambato, Erika Fernanda; Fernández Rivero, Danae
    Considering the growing interest in the utilization of agricultural waste, pineapple peel stands out as a rich source of bioactive compounds. The need to replace synthetic antioxidants and anti-inflammatories, associated with health risks, emphasizes the importance of exploring natural sources. Additionally, the assessment of shelf life is crucial to ensure the stability and efficacy of the extract over time. The extraction process began using ethanol and distilled water as solvents in different proportions. The concentration of polyphenols was determined using the Folin-Ciocalteu method, demonstrating that the ethanol treatment was the most efficient. Total flavonoid evaluation was performed on the extract with the highest polyphenol concentration using the colorimetric method of aluminum chloride. Furthermore, the analysis of antioxidant activity was conducted through the DPPH assay, resulting in an inhibition percentage of 52.27 percent. The analysis of anti-inflammatory activity was also carried out using the egg albumin denaturation assay, revealing significant percentages of denaturation inhibition, reaching 73.15 percent. Stability was analyzed over a month and showed a slight increase in pH and variation in humidity, confirming the robustness of the extract. This study provides valuable information on the shelf life of pineapple peel extract, highlighting its potential as a natural source of antioxidants and anti-inflammatories. This contributes to the expansion of knowledge on the utilization of agricultural by-products and the search for natural solutions to health challenges.
  • No Thumbnail Available
    Item
    Análisis de la actividad antioxidante de un microencapsulado de Mangiferina extraída de la cáscara del mango (Mangifera Indica L.)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Navarro Otavalo, Juan Eduardo; López Hernández, Orestes Darío
    Mango peel, a by-product of mango processing, has been identified as a valuable source of polyphenols. As such, research on the extraction of mangiferin-rich phenolic compounds from this waste peel is of great interest. The mangiferin has attracted scientific attention due to its various applications in health, nutrition and cosmetics, especially for its and cosmetics, especially for its antioxidant capacity. The extraction of phenolic compounds was carried out through response surface methodology, using dehydration, crushing, and infusion with a water-ethanol mixture at various concentrations for different time intervals. The determination of the total polyphenol content (TPC) was carried out using the Folin-Ciocalteu method, achieving a TPC of 93.5928 mg GAE per gram of dry base, with a concentration of 70 percent ethanol. The identification of mangiferin was carried out with UV-Vis and infrared spectrophotometry. The microencapsulation, using a water-extract-maltodextrin mixture, showed an efficiency of 95,146 with a total solids content of 30 percent. The radical inhibition capacity was evaluated by means of the DPPH method, obtaining promising antioxidant promising percentages of antioxidant capacity: 88.373 per cent for the crude extract and 43.165 per cent for the microencapsulated extract. In summary, this study concludes that the microencapsulation process not only overcomes the limitations associated with polyphenols, but also contributes significantly to the increase of the free radical scavenging capacity, highlighting the potential of mango peel in various industrial applications.
  • No Thumbnail Available
    Item
    Evaluación de la actividad antioxidante de polifenoles microencapsulados obtenidos a partir de la cáscara de plátano (Musa paradisiaca)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Montoya López, Camila Victoria; López Hernández, Orestes Darío
    Polyphenolic compounds contain a high antioxidant capacity, which has made them substances of great interest. In Ecuador, there is a large agricultural production of plantain (Musa paradisiaca), but its peels are not used and are only discarded. In this study, we sought to make the most of the plantain peels, determined the best extraction conditions and microencapsulated the polyphenols to analyze their antioxidant activity. With the best ethanol concentration of 30 percent, the influence of the plant material: solvent volume ratio was studied, using ratios of 1:20 and 1:25 and extraction times of 30 and 60 minutes, with the result that the best conditions were 1:25 at a time of 30 minutes. The microencapsulation process was carried out by spray drying at an inlet temperature of 150 degrees Celsius and outlet at 90 degrees Celsius. Maltodextrin was used as polymeric matrix and the microencapsulation efficiency was evaluated by FT-IR spectroscopy. To evaluate the antioxidant capacity, the DPPH method was used, where an inhibition percentage of 82.28 percent was obtained for the extract and 28.50 percent for the microencapsulation.
  • No Thumbnail Available
    Item
    Recuperación de antocianinas a partir de los residuos agroindustriales de la mora de castilla
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Llerena Toasa, Diana Michelle; López Hernández, Orestes Darío
    Tungurahua being one of the provinces with the highest production of blackberry, the idea of recovering anthocyanins from the agroindustrial waste of this fruit, arises as a topic of vital importance, since the bioactive substances that are present in the skin of the blackberry, not only give the characteristic color and flavor to the fruit, but also have antioxidant properties and great benefits for human health. For the extraction of anthocyanins, 96 percent ethanol was used as a solvent, to which citric acid was added to preserve the stability of these bioactives against factors that could affect their integrity. Subsequently, they were microencapsulated with maltodextrin to preserve the activity of the bioactive, thus prolonging their useful life, to finally determine the antioxidant activity of the anthocyanins. It was determined that the optimum conditions to obtain the highest amount of anthocyanins were 1:15 plant material - solvent volume for 30 minutes. In the microencapsulation process, it was obtained an efficiency of 78.178 percent, indicating effective microencapsulation. On the other hand, in the determination of the antioxidant activity, it was obtained 55.606 percent DPPH inhibition in the concentrate (Trolox referent of 311.711 micromol per liter) and 29.342 percent in the microencapsulated (Trolox referent of 205.550 micromol per liter), taking into account that in this value there is only 20 percent of the concentrate. This process represents a significant advance in the valorization of agro-industrial waste and the sustainable production of bioactive compounds.