Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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Formulación de un gel tópico con extracto de flavonoides obtenidos de Artocarpus heterophyllus
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2026-01)
Silva Espinosa, Andrés David
;
Fernández, Rivero Danae
The lack of effective cosmetic products to eliminate free radicals generated by environmental pollutants, temperature changes, and ultraviolet radiation exposure presents an opportunity to utilize waste from plant species, such as Artocarpus heterophyllus, in the development of a topical gel based on flavonoid extracts. In this project, the extraction of the extract was optimized using a two-square factorial design with ultrasound, varying the sonication time and ethanol concentration. Under conditions of 70 percent ethanol for 30 minutes, a higher concentration of flavonoids was achieved, with a value of 1,178 milligrams of quercetin per gram of sample, resulting in an antioxidant activity of 39.62 percent in the DPPH assay, sufficient to consider this extract as a functional ingredient. From this extract, gels were formulated with concentrations of 0.3; 0.6 and 0.9 percent extract were used, and physicochemical tests were performed to evaluate viscosity, pH, and spreadability, to meet USP standards. The resulting gel showed a viscosity of 23,550 cP, a pH of 5.31, and a spreadability of 5.9 cm, values that are within the acceptable range for topical applications. The formulation with 0.9 percent extract was the only one capable of exhibiting antioxidant activity of 19.37 percent, while the lower concentrations showed no significant changes, indicating that an adequate amount of extract is necessary to obtain an appreciable antioxidant effect.
Elaboración de una crema antioxidante a partir de flavoniodes extraídos de las hojas de verbena (Verbena litoralis Kunth)
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-07)
Pinto Franco Jennifer Nicole
;
Fernández Rivero Danae
The lack of knowledge about the bioactive properties of traditional plant species represents a significant loss of natural resources with potential added value. In Ecuador, Verbena litoralis Kunth, a plant of ancestral use, has been underutilized despite its high content of flavonoids with antioxidant properties. In response to this problem, the present study proposed the elaboration of an antioxidant cream from flavonoids extracted from verbena leaves with potential application in the natural cosmetic industry. To obtain the active compounds, an experimental design with three factors was used: mass/solvent ratio, extraction time and temperature. The flavonoid content was evaluated using a spectrophotometric method and the antioxidant capacity of the extract was determined using the DPPH assay. The optimum treatment corresponded to mass-solvent ratio (2:10), extraction time (30 minutes) and temperature of 50 Celcius degrees obtaining a flavonoid content of 32.508 milligram equivalents of quercetin per ml of extract and an antioxidant capacity of 77.74 percent inhibition. Subsequently, the extract with the highest activity was incorporated into a cosmetic base for the preparation of an antioxidant cream, which was subjected to physicochemical and organoleptic evaluations. The results obtained show that Verbena litoralis has a high potential as a source of bioactive compounds, which allows its application in natural cosmetic products, promoting the use of traditional plants and sustainable innovation in skin care.
Evaluación de la producción de fenoles, flavonoides y antioxidantes en sistemas biotecnológicos de pitahaya (Hylocereus megalanthus) con metil jasmonato
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08)
García Ramos, Marissa Miossothy
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Lalaleo Córdova, Liliana Paulina
This research evaluates the effects of methyl jasmonate as a plant stress inducer on the production of phenolic compounds, flavonoids and antioxidant capacity in cellular biotechnological systems of pitahaya (Hylocereus megalanthus). Stress was induced at different concentrations of 0, 50 and 150 micromolar methyl jasmonate at time intervals of 7, 14, 21 and 28 days. The results showed with 50 micromolar a continuous maximum phenol production until day 28 with 137,639 equivalents of gallic acid, while 150 micromolar achieved a phenol production of 126,087 equivalents of gallic acid at 28 days. In flavonoid production, 150 micromolar showed the fastest increase, 121,562 quercetin equivalents in 14 days. In antioxidant capacity, 150 micromolar MeJa elicited the highest initial response, 89.518 percent inhibition of DPPH within 14 days. This study concludes that, for industrial applications aimed at both growth and the production of bioactive compounds, the treatment of pitahaya cells with 50 micromolar methyl jasmonate is optimal, since they provide a balance between progressive growth and greater production of metabolites. Alternatively, higher concentrations such as 150 micromolar methyl jasmonate can be used for rapid compound production despite reduced cell growth. The findings provide valuable information for laboratory processes at industrial levels, depending on specific production objectives and as a contribution to the limited research of these compounds in plant cell suspensions.
Evaluación del contenido de fenoles, flavonoides y fructosa de una bebida a partir de la hoja y el fruto del nopal (Opuntia ficus-indica)
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Alimentos, 2023-09)
Oña Oña Verónica Lisbeth, Verónica Lisbeth
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Paredes Escobar, Mayra Liliana
Diabetes is a chronic disease, currently presents a great challenge for its treatment and food suitable for this sector of the population. The nopal has excellent benefits that can be incorporated into healthy diets, its high concentrations of calcium, potassium, magnesium and minerals help in the bone system, its content of phenols, flavonoids, fructose and vitamin C, help in the control of high cholesterol, blood glucose levels, among others, however, is not exploited in Ecuador. This research project characterized the physicochemical properties of Ecuadorian nopal, developed an acceptable nopal beverage through sensory analysis and evaluated the phytochemical content of phenols, flavonoids and fructose by UV-VIS spectrophotometry in the developed product. For leaf and fruit, titratable acidity was 0.13 and 0.27, vitamin C was 23.6 and 22.9, Brix was 0.91 and 11.36 with a pH of 6.19 and 4.61 respectively. The result of the sensory analysis of the formulation by both extraction methods was Samples 1 (50T50N), being the most acceptable by the 40 tasters. The phenol content of the pulped and pressed beverages was 106.99 and 101.69 mgGAE per-L respectively, both samples showed significant phenol content. The flavonoid content of the pulped and pressed beverages was 18.88 and 17.19 mg QE per-L, and finally, the pH was 6.66 for the pulped beverage and 6.65 for the pressed beverage, indicating that they tend to be slightly acidic.
Extracción y caracterización de metabolitos secundarios (polifenoles y flavonoides) obtenidos a partir de Pujín (Hesperomeles ferruginea) planta nativa del cerro Teligote
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería en Alimentos, 2020-08)
Rodríguez Barriga, Karina Alexandra
;
Morales Acosta, Dayana Cristina
In the Pelileo canton belonging to the province of Tungurahua is the Teligote hill, whose biodiversity has been preserved for decades by the Salasaca culture. One of the representative species is the Pujín (Hesperomeles ferriginea), native plant of the hill and base of the study. In the present, the extraction process of secondary metabolites (polyphenols and total flavonoids) was optimized, evaluating factors such as the type of solvent, temperature, time and number of successive extractions. For the quantification of polyphenols, the Folin-Ciocalteu method and calibration curve with gallic acid were applied, the conditions established for the extraction were methanol at 50 percent, 50 degrees centigrade for 5 min with 4 extractions, obtaining as a result 79.541mg EAG about g of more. While for the quantification of flavonoids, the aluminum chloride method and catechin calibration curve were applied, resulting in the optimal conditions for the extraction, 25 percent methanol at 50 degrees centigrade, 5 min with 3 extractions, obtaining 49.751 mg ECQ on g of more., in both cases reaching a yield greater than 95 percent; It was found that the plant matrix has a high number of polyphenols and flavonoids in relation to other matrices. Complementary to this, the characterization of secondary metabolites was carried out using chromatographic methods (HPLC and TLC), obtaining chromatograms that allowed verifying the presence of polyphenols and flavonoids, in addition to observing that the plant matrix contains a wide spectrum of compounds of interest at different wavelengths.
Caracterización de extractos obtenidos a partir de Tarqui Myrsine andina (Mez) Pipoly, planta nativa del cerro Teligote
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería en Alimentos, 2021-01)
Castillo Villamarín, Jeniffer Gabriela
;
Morales Acosta, Dayana Cristina
The objective of this research work was to characterize the extracts obtained from TARQUI Myrsine andina (Mez) Pipoly, a native plant of Cerro Teligote, for the optimization of the extraction process, three factors were considered: solvent (ethanol, methanol, at a concentration of: 25, 50 and 75 percent and pure) and water; temperature (30, 40, 50, and 60 degrees centigrade) and time (5.10 and 20 min), the extraction method was standardized for polyphenons, 50 percent ethanol, 50 degrees centigrade and 5 min, while for flavonoids it was 75 percent ethanol, 50 degrees centigrade and 20min. When comparing the different solvents for the extraction of polyphenols, the highest percentage of plant wear was obtained which was 98.91 percent for flavonoids and 97.74 percent for polyphenols and thus obtain the most concentrated extract with the minimum degradation of its compounds during the production process. The concentration of polyphenols in the tarqui extracts was determined by the Folin Ciocalteu method, and the ultraviolet-visible spectrophotometry method was used for the concentration of total flavonoids. The separation and identification of the polyphenols and flavonoids present in the extract, high performance liquid chromatography (HPLC) with two standards was used.
Obtención y caracterización de polifenoles y flavonoides de extractos de Baccharis macrantha (Chilca) y estudio de su actividad antioxidante y antiinflamatoria
(2020-01)
Rosero Mero, Santiago André
;
Morales Acosta, Dayana Cristina
The aim of this study was to obtain the highest content of total flavonoid (TFC) and total polyphenol (TPC) from dry leaves of Baccharis macrantha, for which an extraction method was optimized using different solvents (ethanol and methanol), temperatures (30-60 Celsius degrees) and extraction times (5-20 min). The optimized conditions for flavonoids extraction were 25 percent ethanol, 50 Celsius degrees and 10 min, while for polyphenols were 50 percent ethanol, 50 Celsius degrees and 10 min; under these conditions depletion of plant material was achieved with recoveries of 98.56 percent for flavonoids and 100 percent for polyphenols in the fourth extraction. The extracts were characterized by TLC and HPLC with three standards (gallic acid, catechin and quercetin). Thus, quercetin was detected in hydroethanolic extracts with an Rf of 0.62 and a retention time of 27 min. In TLC, 9 bands were also detected, with apparently methylated and glycosylated groups, while in HPLC 14 representative peaks were detected that share both extraction treatments for each wavelength used. The biological activities were tested in vitro. Anti-inflammatory activity was evaluated using the protein denaturation method and red blood cells stabilization, whose protection values were 93.70 percent (EtOH50 percent) and 97.23 percent (EtOH25 percent); and 49.72 percent (EtOH25 percent) and 50.71 percent (EtOH50 percent), respectively. The antioxidant activity evaluated through the TBARs method, showed a protection equal to 77 percent (EtOH25 percent) and 73.11 percent (EtOH50 percent).
Estudio del contenido de polifenoles, capacidad antioxidante y antiinflamtoria del extracto obtenido de tipo (Minthostachys mollis Griseb)
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2020-01)
Muñoz Jumbo, Diana Lizbeth
;
Morales Acosta, Dayana Cristina
The present research work is focused on the extraction of secondary metabolites, specifically phenolic compounds present in the tipo leaves (Minthostachys mollis Griseb) and the evaluation of their biological activities. Tipo plant extract was optimized by different factors (solvent, temperature, time and number of successive extractions) of these, methanol 50 percent, 50 Celsius degrees, 10 min with 4 successive extractions presented the optimal conditions to obtain the highest polyphenol and flavonoids content which was 70,115 more less 0.440 mg gallic acid equivalents per gram dry sample and 64.967 more less 0.858 mg catechin equivalents per gram dry sample, respectively. Two biological activities were evaluated, the antioxidant activity by the thiobarbituric acid reactive substances (TBARS) method where the highest percentage of inhibition 68.987 percent was obtained at 1000 ug per mL. The anti-inflammatory activity was evaluated by two methods: a) protein denaturation and b) human red blood cell membrane stabilization (HRBC) that presented an important percentage of protection (94.398 percent). Finally, the content of minerals present in tipo leaves was evaluated by atomic absorption spectrophotometry and a high content of Ca and Mg was obtained (13174.749 mg per kg dry base and 5771.208 mg per kg dry base, respectively). Due to the presence of these biological activities, tipo could become a natural alternative that substitutes synthetic products and contributes to the treatment of various pathologies, in addition to providing essential nutrients to the human being.
Extracción, cuantificación y caracterización de compuestos fenólicos extraídos a partir de la Arquitecta (Lasiocephalus ovatus Schltdl), planta nativa del cerro Teligote
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2020-01)
Espinosa Panchi, Melany Noemí
;
Morales Acosta, Dayana Cristina
L. ovatus is a native plant from Teligote’s hill (1°22'39.2"S; 78°33'54.7"O; 3633 m.s.n.m.). It has used by locals people as diuretic and depurative. The aim of this research study was extract, quantify and characterize phenolic compounds obtained from L. ovatus. Dried and crushed leaves were used from the first step of optimization processed that consist of choosing the major solvent (water, methanol and ethanol), time (5, 10, 20 min) and temperature (30, 40, 50, 60 Celsius degrees). The best conditions to extract polyphenols in L. ovatus matrix were ethanol 50 percent (v/v), 50 Celsius degrees and 20 min, while by flavonoids were ethanol 50 percent (v/v), 60 Celsius degrees and 5 min. Depletion of vegetal material was made with 4 and 5 successive extractions for polyphenols and flavonoids, respectively. TLC and HPLC techniques were used to characterize phenolic compounds in the extracts of L. ovatus. Furthermore, anti-inflammatory activity was tested in vitro by HRBC obtained of were 98,04 more less 0,44 for flavonoids and 97,29 more less 0,12 for polyphenols and protein denaturation method with values of 36,30 more less 0,29 for polyphenols and 28,71 more less 0,63 for flavonoids. Antioxidant activity showed values 86,46 more less 0,83 y 84,18 more less 0,57 for polyphenols and flavonoids respectively. In this way it was concluded that L. ovatus presents in its structure phenolic components that confer important biological activities (anti-inflammatory and antioxidant activity) thus supporting its use inside and outside the community of Teligote as a possible natural alternative.

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