Carrera de Biotecnología

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34800

Browse

Filters

2022 - 20255

Settings

Subject: search.filters.subject.POLIFENOLES

Search Results

Now showing 1 - 5 of 5
  • No Thumbnail Available
    Item
    Evaluación de la vida útil de un extracto con polifenoles procedentes de la flor de Jamaica (Hibiscus sabdariffa L.) obtenido mediante extracción asistida por ultrasonido
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Aldás Loma, Emily Vanessa; Fernández Rivero, Danae
    The focus on the use of natural products has grown considerably in recent years due to their therapeutic benefits, including antioxidant and anti-inflammatory activity. Plant extracts represent an important source of bioactive compounds, such as polyphenols, which contribute directly to these properties. However, to ensure the efficacy and quality of these extracts, it is essential to determine their shelf life, since factors such as pH, humidity and storage temperature can influence the stability of these compounds. In this study, the Jamaican flower extract was obtained by ultrasound-assisted extraction and the concentration of polyphenols was determined by the Folin-Ciocalteu method, achieving a higher polyphenol content when using an ethanol concentration of 70 percent and with an extraction temperature of 50 degrees Celsius, reaching a value of 62,667 milligrams of gallic acid per gram of dry weight. Subsequently, its antioxidant capacity was evaluated through the DPPH assay, obtaining 90.886 percent inhibition, and with respect to Trolox equivalents, 221.234 micromoles per gram of dry weight were obtained. The antioxidant activity was analyzed by egg albumin denaturation assay, registering inhibition percentages between 38.642 and 62.585. Finally, the shelf life, stored in amber glass jars under refrigerated conditions, was determined to be about 32 days, fitting a first-order kinetic model with a rate constant of 0.0032 per day.
  • No Thumbnail Available
    Item
    Evaluación de la capacidad antioxidante y antiinflamatoria de polifenoles microencapsulados procedentes de los residuos de tamarindo (Tamarindus indica)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Mendoza García, Damaris Katiusca; López Hernández, Orestes Darío
    Tamarindus indica is a fruit widely used in the production of various commercial products, generating a large amount of agroindustrial waste per year. Tamarind seeds represent a source for obtaining bioactive compounds of interest such as polyphenols. The present study seeks to take advantage of these wastes by determining the optimal conditions for extraction and microencapsulation of polyphenols to evaluate their antioxidant and anti-inflammatory capacity. The influence of extraction time and solvent concentration on obtaining polyphenols was studied. The best extraction conditions were at a solvent concentration of 53 percent for a time of 80 minutes, providing a polyphenol content of 516.521 mg GAE per gram of plant material. Spray-drying microencapsulation using maltodextrin as the encapsulating agent evidenced a percentage efficiency of 87.066 percent. The efficiency of microencapsulation was also evaluated by means of infrared spectroscopy (FT-IR). The DPPH method was applied to establish the antioxidant potential of the extract and microencapsulation, reaching values of 80.085 percent and 48.916 percent, respectively. Finally, the anti-inflammatory activity was determined through the percentage of inhibition of the denaturation of egg albumin and bovine serum albumin (BSA), obtaining inhibition percentages in each of the concentrations studied. The results obtained in this research show the potential of tamarind residuals with biological activities and their possible application in different industries.
  • No Thumbnail Available
    Item
    Evaluación de la capacidad antioxidante de los polifenoles microencapsulados procedentes de la cáscara del taxo (Passiflora tarminiana)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Lema Ochog, Mónica Alexandra; López Hernández, Orestes Darío
    Taxo peel is a by-product from the drying and peeling activities of the fruit processing industries. These residues, when not properly treated, end up in landfills generating environmental pollution problems that are hazardous to health, completely avoiding their polyphenolic content. Therefore, the extraction of these bioactive compounds is of interest for future applications in the food, cosmetic or pharmaceutical industry. For the extraction of polyphenols, two factors were studied, the time of 30, 60 and 90 minutes and the concentration of the solvent (EtOH) of 25, 50 and 75 percent, being 66,37 minutes with an amount of solvent at 58,99 percent the optimum conditions to maximize the polyphenolic content, with which, a total of 130,69 milligrams of gallic acid equivalents per gram of dry base was obtained. Subsequently, for the microencapsulation technique by spray drying, an inlet and outlet temperature of 150 and 90 degrees Celsius was used, respectively, with maltodextrin as the encapsulating agent, reflecting an efficiency of 83,50 percent. Finally, antioxidant activity was determined by the DPPH test, with an inhibition percentage of 80,04 for the concentrated extract and 20,10 for the microencapsulated one. It is concluded that the microencapsulation technique keeps the bioactive compounds stable and helps to release them in a controlled manner. The result is useful in the formulation of nutraceutical products important for health and disease prevention.
  • No Thumbnail Available
    Item
    Evaluación de la actividad antifúngica de un microencapsulado rico en polifenoles del residuo de caña de azúcar (Saccharum officinarum L) frente a Botrytis cinerea
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Abril Díaz, Viviana Gabriela; López Hernández, Orestes Darío
    Currently, biotechnology is looking for alternatives to replace commercial fungicides in the control of pathogens such as Botrytis cinerea, responsible for approximately 10 percent of annual crop losses. Research on the use of plant residues, whose metabolites fulfill this purpose without causing harm to the environment or humans, have emerged as central areas of study. Such is the case of sugarcane bagasse, rich in polyphenols with antifungal activity, being capable of being used in the future within the fields as a pest control agent. To obtain the extract rich in polyphenols, the organic solvent extraction method was used using ethanol at 30, 50 and 70 percent evaluated at 30, 45 and 60 minutes in a 1:20 plant material:solvent ratio. After this, the Folin test was performed to measure the concentration of total polyphenols, the best treatment being 50 percent at 30 minutes for 63 g per GAE. Next, microencapsulation of the concentrate was carried out at a 20:80 ratio using maltodextrin, using the spray drying technique, and its efficiency was evaluated using FTIR. Finally, the in vitro antifungal activity was evaluated at 10, 30 and 50percent microencapsulation as an antibiogram, obtaining a higher percentage of inhibition at 50 percent microencapsulation than the total average of the 8 days of the trial.
  • No Thumbnail Available
    Item
    Obtención y microencapsulación de un extracto rico en compuestos fenólicos con capacidad antioxidante de guayusa (Ilex guayusa)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2022-09) Peñaloza Suárez, Adriana Gabriela; López Hernández, Orestes Darío
    Phenolic compounds are bioactive molecules whose properties, such as their antioxidant capacity, have made them substances of great interest in different industries. In recent years, guayusa (Ilex guayusa) has been studied as an important source of these compounds. Therefore, in this study, the best conditions for extraction, microencapsulation, and antioxidant activity of phenolic compounds from guayusa leaves were determined. A three-square experimental design was applied in which the solvent concentration (ethanol) and the extraction time were evaluated to optimize the obtaining of phenols. The best extraction conditions indicated by the surface analysis were with 42 percent ethanol for a time of 62 minutes. In the microencapsulation process, the following variables were studied: extract concentration and inlet temperature. The optimized microencapsulation conditions obtained were a concentration of 30 percent solids of the extract at an inlet temperature of 150 degrees Celsius. Finally, for the evaluation of the antioxidant capacity, the DPPH method was applied, obtaining as a result a higher percentage of inhibition for the liquid extract than for the microencapsulated one with higher efficiency.