Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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2014 - 201922020 - 20263

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  • Miniatura
    Item type:Ítem,
    Evaluación de la producción de luteína en el cultivo de Chlorella vulgaris bajo condiciones de laboratorio
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2026-07)
    Lascano Chango, Darwin Alexander
    ;
    Fernández Rivero, Danae
    Currently, interest in microalgae has increased due to their potential as a source of bioactive compounds that can be applied in functional foods and nutraceutical supplements. Research on pigments produced by these microorganisms is highly relevant. For example, lutein has antioxidant properties that are beneficial for eye health, and its importance in biotechnology has generated interest in its production under controlled conditions. In the present study, experimental cultures of Chlorella vulgaris grew in standard BBM medium and in medium with nitrogen, phosphorus, and potassium concentrations reduced by fifty percent, under low and high light intensities: two thousand six hundred thirty-eight lux and five thousand two hundred sixteen lux, respectively. Cell density was monitored for twenty-two days to determine the best treatment, with the aim of adding two promoters under that condition for lutein production: sodium acetate and acetic acid. This carotenoid was quantified using spectrophotometric methods with ninety-six-well microplates. The results showed that cultures grown in standard medium under high light intensity reached the highest cell densities and showed a prolonged exponential phase. Lutein production was higher in treatments with an organic carbon source, although cultures without promoters also presented detectable amounts of lutein. The combination of a balanced medium and controlled light conditions proved to be efficient for maximizing biomass and the content of bioactive pigments.
  • Miniatura
    Item type:Ítem,
    Uso de la metabolómica para verificar autenticidad, origen y aporte nutracéutico entre cultivares de Tropaeolum tuberosum
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03)
    Hernández Salazar, María José
    ;
    Lalaleo Córdova, Liliana Paulina
    The present study consisted of the processing and analysis of data from a metabolomics study using the nuclear magnetic resonance technique of six different cultivars of Tropaeolum tuberosum (mashua), whose results are published by Valle-Parra et al 2020: From morphological traits to the food fingerprint of Tropaeolum tuberosum through metabolomics by NMR. Data includes: cultivar ID (A-F); sample collection area (I, II and III); information on the content of 34 metabolites (in mM); and integration values of each resonance spectrum at an interval of 0.4 ppm (234 values per spectrum). These data were subjected to multivariable analysis by PCA revealing a grouping between cultivars A-B, C-D-E and F. These three subgroups coincided with the grouping of the analysis of the collection area. Subsequently, the performance of the machine learning classification models was evaluated: LDA, SVM and RF. The former had the lowest performance with a percentage accuracy range between 83.2 and 87.6, the second between 86.5 and 96.4, and RF had the best performance between 96.4 and 100. Finally, the nutraceutical contribution of the cultivars was evaluated, and they were ranked according to their content of amino acids, carbohydrates, organic acids, among other nutrients. In global and amino acid terms, cultivar F stood out for its superior contribution to the other cultivars; cultivar A has the highest content of organic acids, while cultivars B and D of carbohydrates.
  • Miniatura
    Item type:Ítem,
    Determinación de la velocidad máxima de transferencia de oxígeno y la velocidad específica máxima de consumo de oxígeno en cultivos por lote de la bacteria (Bacillus subtilis DS23) en un biorreactor
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2020-01)
    Robalino Travez, Daniela Estefanía
    ;
    Cerda Mejía, Liliana Alexandra
    This research was carried out with the purpose of optimizing the production of biosurfactant with amphipathic activity from Bacillus subtilis DS23, which has been characterized as one of the microorganisms that have the best performance when producing this type of compounds. The experimentation was carried out in a 5-liter bioreactor maintaining constant parameters such as pH at 6.5, temperature at 30 Celsius degrees and aeration in 1 volume of air per volume of medium per minute (vvm), working with speeds of agitation of 150, 200 and 300 revolutions per minute (rpm) being this the only parameter that was varied. It was shown that the production of biomass and bio-surfactant in a stirred tank bioreactor is strongly influenced by agitation. The best results of both biomass and biosurfactant were obtained at 300 rpm, agitation speed that was used to determine the parameters proposed in this investigation. Using the Humphrey dynamic method using air as an oxygen-carrying gas, the effect of dissolved oxygen in the medium on the growth and production of biosurfactant was assessed by estimating the volumetric oxygen transfer coefficient (KLa), the specific rate of oxygen consumption (qO2) and the oxygen transfer rate (VTO) obtaining 0.002 h-1, 0.079 mmol O2 for L * h and 0.312 h-1 respectively. It was decided to evaluate these parameters because they are key in aerobic crops, and they are intended to serve as scaling criteria in future research.
  • Miniatura
    Item type:Ítem,
    Determinación de la biodiversidad y distribución geográfica de levaduras asociadas a frutos de mora de castilla (Rubus glaucus) de tres localidades de la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería en Alimentos, 2014)
    Cevallos Campaña, Cristina Beatriz
    ;
    Rodríguez Meza, Carlos Alberto
    Ecuador reports a few information about yeast biodiversity, most of the microbiological research are focused to bacteria. Fruits in their natural environment represent important habitats for yeasts. The target of this research was the determination of the diversity of yeasts as sociated to blackberry fruits from Salasaca, Cevallos and Tisaleo, where organic blackberries are cultivated. The sector with greater diversity was Tisaleo where 6 species were isolated, followed by Salasaca where 4 species were isolated and finally Cevallos, where only 2 species were isolated. A traditional characterization of the isolates was made, it consisted in macroscopic characterization where optical characteristic of colonies were evaluated such as: margin, elevation, surface, brightness, texture and color. A microscopic characterization in which was showed the morfology and the size of yeast cells. A physiologycal characterization with 8different carbon sources (dextrose, xylose, maltose, galactose, lactose, sucrose, cellobiose and soluble starch), assimilation and fermentations characteristics were evaluated for each of the yeast isolates. However, those techniques were not enough to achieve the identification of the isolates to the taxonomic level of genre or specie, the study of each isolated strain was complemented by the use of molecular techniques based on the restriction analysis of the gen rDNA 5.85S and the internal transcripted spacers (ITS1 and ITS2), getting 9 restriction profiles. The resultant profiles were compared with the ones from the data base of the Quito Católica Yeast Collection CLQCA (Acronym in Spanish). 5 profiles were identified by this technique as Meyerozyma guilliermondii, Hanseniaspora meyeri, Whickerhamomyces onychis,Galactomyces geotrichum and Pichia manshurica. The remaining yeasts were not identified bythis technique because they did not match similar band patterns with the ones from the CLQCA database. The taxonomic status of these cultures was determined by 26S rDNA sequencing giving as result: three isolates of Clavispora lusitaneae, two isolates of Metschnikowia pulcherrima one corresponding to Candida sorbosivorans and two corresponding to Pichia membranifaciens. The data obtained in this research demonstrate the great diversity of yeast associated to blackberry fruits, providing basic knowledge to find potential biotechnological applications.
  • Miniatura
    Item type:Ítem,
    Determinación del potencial antimicrobiano de la microalga Chlorella extraída de las aguas empozadas de las acequias de Atocha, Tilulum y el Socavón del cantón Ambato
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2014)
    Barona Altamirano, Adriana Carolina
    ;
    Valencia Silva, Alex Fabián
    Las microalgas son organismos unicelulares eucariotas fotosintéticos capaces de transformar la energía luminosa en energía química; las condiciones óptimas de crecimiento varían de una especie a otra, las investigaciones sobre el cultivo de microalgas son de gran importancia dada su amplia aplicación biotecnológica y comercial. Chlorella es una microalga verde unicelular de agua dulce, de forma esférica mide cerca de 2-10 micrones de diámetro, tiene alta concentración de clorofila, se reproduce rápidamente y de forma asexual, necesita carbono agua luz y pequeñas cantidades de minerales.Se recolecto diferentes muestras de aguas empozadas de Atocha, Tilulum y el Socavón de la ciudad de Ambato, los cultivos de la microalga Chlorella se aislaron utilizando el medio Basal de Bold debido a la cantidad de nutrientes que posee, las células tardaron 240 horas en alcanzar la fase exponencial para su posterior análisis. Se determinó el potencial antimicrobiano de la microalga en base a los resultados positivos en los ensayos en los que se utilizó el precipitado que resulta de la extracción del chlorellin con los solventes etanol y alcohol isopropílico. El análisis estadístico determinó que el mejor tratamiento fue Tilulum- Alcohol isopropílico al tener mayor el tamaño del halo de inhibición, cuya media fue de 28mm, ante la bacteria coco grampositiva, mientras que el menor tratamiento fue Socavón-etanol con una media de 0,11mm. En la última etapa de la investigación se realizó las pruebas de control positivo mediante la técnica del antibiograma utilizando la bacteria aislada y antibióticos como penicilina, demostrando que fue sensible con un halo de inhibición de 5mm ante la ampicilina y 1mm ante oxacilina, valores que están dentro del rango de sensibilidad de acuerdo a las tabla CIM (Concentración Mínima Inhibitoria).