Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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Fecha de inicio: 2020Tema: search.filters.subject.CELULOSA BACTERIANA

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  • Miniatura
    Item type:Ítem,
    Evaluación del efecto del ácido tereftálico en la producción de celulosa bacteriana por Komagataeibacter xylinus DSM2004
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Alimentos, 2026-07)
    Herrera Bermudes, Erick Ricardo
    ;
    Cerda Mejía, Liliana Alexandra
    The industrial-scale production of bacterial cellulose (BC) faces limitations associated with low yields and the high costs of conventional substrates, which justify the exploration of alternative additives such as terephthalic acid (TPA), derived from PET recycling. This study evaluated the effect of TPA supplementation on BC production using the strain Komagataeibacter xylinus DSM2004. The study analyzed the interaction of three critical factors: pH, TPA concentration, and cultivation type. The results indicated that the optimal condition was pH 6.5 with 1 percent TPA under static cultivation, achieving a yield of 4.62 g per L. Supplementation with 1 percent TPA enhanced yield compared to the conventional medium; however, increasing the concentration to 2 percent resulted in an inhibitory effect, particularly under agitated conditions, where glucose consumption ceased prematurely. FTIR characterization showed that TPA did not produce detectable alterations in the functional group’s characteristic of bacterial cellulose. Mechanical analysis revealed that the presence of the monomer influenced the flexibility and stiffness of the material, suggesting a possible plasticizing effect. Overall, the results suggest that the valorization of TPA derived from PET recycling may constitute a technically viable strategy for the production of advanced biomaterials, integrating circular economy principles into biotechnology.
  • Miniatura
    Item type:Ítem,
    Caracterización de la celulosa bacteriana producida a través de combinaciones de Komagataeibacter xylinus DSM2004 y los consorcios PDC21 y PDC25
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08)
    Tiban Mullo, Bibiana Liseth
    ;
    Cerda Mejía, Liliana Alexandra
    Bacterial cellulose is a biopolymer of great commercial interest due to its high purity, high degree of crystallinity and superior mechanical properties compared to cellulose derived from vegetable sources. However, its high production cost has limited its use on an industrial scale. To reduce these costs, it is essential to explore techniques that optimize cellulose biosynthesis, such as the use of co-culture methods that increase the robustness of the production system and enhance the yield through synergistic interactions. In the present study, bacterial cellulose produced by combinations of a standard strain and the microbial consortia PDC21 and PDC25 was characterized, evaluating the production in co-cultures versus monocultures. Cellulose morphology was analyzed using scanning electron microscopy (SEM), chemical composition by Fourier transforms infrared spectroscopy (FT-IR) and crystallinity was evaluated by X-ray diffraction (XRD). Additionally, the resulting fermentation broths were characterized to understand the differences between co-culture and monoculture productions. The co-culture treatments demonstrated higher cellulose yields compared to their monocultures. Although all treatments exhibited similar chemical characteristics, variability was observed in the morphology and crystallinity of the cellulose produced. Significant differences were also identified in the physical and chemical characteristics of the fermentation broths. This study evidenced the potential of bacterial co-cultures to improve cellulose production efficiency.
  • Miniatura
    Item type:Ítem,
    Obtención y caracterización de celulosa bacteriana producida por cepas acéticas endémicas del Ecuador
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09)
    Álvarez Morales, Juan José
    ;
    García Solís, Mario Daniel
    The research and development of new bacterial strains producing bacterial cellulose (BC) is a topic of great relevance today, especially in the context of biotechnology and industrial applications. This study focuses on native strains from Ecuador, with special attention to acetic bacteria PDC 21 and PDC 25, opening new possibilities for BC production. The methodology of this study involved the axenic culture of the strains and the observation of the formation of BC biofilm. Fermentative processes were carried out and the BC production yield was evaluated in comparison with a control of Komagataeibacter xylinus (DSMZ 2004). In addition, acid, thermal, and mechanical treatments were performed to obtain nanocellulose. The morphology of the samples was analyzed by scanning electron microscopy (SEM), while Fourier Transform Infrared Spectroscopy (FTIR-IR) was used to evaluate their chemical properties. The study concludes with the proposal that strain PDC 25 could be a viable alternative for BC production in industrial applications. This conclusion is based on its production yield, glucose consumption, and the properties of the BC it produces, which are similar to those of strain DSMZ 2004, commonly used as a study model and in the industry. This finding opens new possibilities for BC production and biotechnology in Ecuador.
  • Miniatura
    Item type:Ítem,
    Análisis in silico de las rutas metabólicas necesarias para la síntesis de celulosa bacteriana en Komagataeibacter xylinus a partir de lactosa
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-09)
    Zapata Berrones, Karina Elizabeth
    ;
    García Solís, Mario Daniel
    Bacterial Cellulose symbolizes a multifaceted resource of exceptional capabilities and fascinatingproperties. Currently, the demand for this exopolysaccharide has progressively increasing, in function of the optimization of processes and the implementation of agro-industrial waste as alternative carbon sources. In such a context, the archetype productor (K. xylinus) represents one of the most valuable organisms in the biotechnological field, a scenario that has made it possible to demonstrate its enormous power to synthesize BC from a wide range of substrates. However, some authors have elucidated its inability to metabolize lactose, caused by the lack of genes responsible for the transport and catabolism of this substrate. Here, DSM 2004 and the analogous strain E25 (identified by phylogenetic analysis), were studied with the aim of identifying missing genes and minimal genetic equipment. A critical analysis of metabolic pathways in BAL and BAA was carried out to list the transporter and the enzymes involved in the process of interest. In this, the summarized proteins were evaluated according to kinetic parameters, where the best results report access code and both peptide and nucleotide sequence of the gene that encodes them. In this, the genes of Escherichia coli K-12 (Lactose permease), Alicyclobacillus acidocaldarius ATCC 27009 (β – galactosidase), Bifidobacterium longum subsp. Infantis ATCC 15697 (galactokinase), and Escherichia coli XL1-Blue (galactose-1-P-uridyltransferase) were selected. Finally, the modeling of the new biosynthetic route presented two related modules: i) Transport and hydrolysis of Lactose and ii) Biosynthesis of CB, after the total replacement of glucose by whey lactose.
  • Miniatura
    Item type:Ítem,
    Aislamiento y caracterización de cepas nativas de Komagataeibacter xylinus y comparación de su crecimiento en diferentes sustratos
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03)
    Dávalos Cerrón, Patricia Yissel
    ;
    Cerda Mejía, Liliana Alexandra
    Komagataeibacter xylinus is the most efficient species, being able to synthesize bacterial cellulose biofilms (CB) from a great variety of carbon sources. This biopolymer has received considerable attention on the last few years due to diverse commercial applications, especially in the food industry and biomedical due to its high purity and variety in form and texture. In the present study, native strains of K. xylinus were isolated and characterized from acetic sources such as wine, wine vinegar and kombucha SCOBY in the media H-S and GYC comparing its production with the strain from control K. xylinus DSM 2004 in the different carbon sources (glucose, whey and ethylene glycol) in static cultivation. Two strains were isolated from the kombucha SCOBY. The results revealed that both strains denominated PDC 21 and PDC 25, and the control strain synthesized CB in different amounts from the glucose, ethylene glycol and fermented milk serum. The strain PDC 25 produced almost 8 more times CB than the strain control in the standard H-S medium compared with PDC 21 that only synthesized 0.4 times the CB humid, meanwhile with ethylene glycol PDC 25 synthesized 1.4 and 3.46 more times CB in the media H-S ethylene glycol and K- ethylene glycol respectively compared by the control strain. However, K xylinus DSM 2004 produced larger amounts of CB in the media that contained fermented milk serum.
  • Miniatura
    Item type:Ítem,
    Análisis filogenético de Komagataeibacter xylinus K2G30=UMCC2756 para la producción de biofilms a partir de suero lácteo
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería en Alimentos, 2021-01)
    Balseca Mora, Evelyn Karina
    ;
    Cerda Mejía, Liliana Alexandra
    Bacterial cellulose, being an organic, renewable, and biodegradable biopolymer has received considerable attention. Here, the production of bacterial cellulose by Komagataeibacter xylinus K2G30 (UMCC 2756) was studied in static culture using Hestrin and Schramm (HS) medium with total replacement of glucose by whey and lactic acid. Furthermore, phylogenetic trees were made from sequences of the 16S ribosomal RNA gene from the species K. xylinus, K. europaeus, K. hansenii and K. intermedius, which revealed that they lacked the lac operon responsible for lactose hydrolysis. Due to these characteristics, they can explain that the highest production of BC by the K. xylinus K2G30 strain was achieved with the use of lactic acid as the sole carbon source. Results provide important information for future studies on the synthesis of BC from different carbon sources, including raw materials from industrial waste such as whey, which must be previously fermented for better assimilation.