Ciencia e Ingeniería en Alimentos y Biotecnología

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Subject: search.filters.subject.ENTEROBACTERIAS

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    Perfiles de la resistencia antimicrobiana de enterobacterias aisladas a partir de comida callejera de la ciudad de Ambato
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Maestría en Ciencia de los Alimentos, 2022-05) Tubón Ocaña, Jessica Magaly; Calero Cáceres, William Ricardo
    Consumption of ready-to-eat street food in Ecuador is extensive, and the information about the presence of foodborne pathogens, their virulence factors, and antimicrobial resistance is negligible. Foodborne pathogens represent a significant cause of negative impacts on human health and the economy worldwide. This investigation includes the occurrence, antibiotic resistance profiles, and antibiotic resistance genes of Enterobacterales isolated from ready-to-eat street foods in Ambato, Ecuador, between 2020 and 2021. The results highlight the high frequency of detection of positive isolates of enteric microorganisms such as E. coli, Klebsiella, Salmonella, Cronobacter, Morganella, and Rahnella. Among the E. coli isolates, only one microorganism pertains to the enterohemorrhagic o enteropathogenic pathotype (EPEC o EAEC). The profiles of phenotypic and genotypic resistance to antibiotics show high percentages of resistance to antibiotics of clinical interest, such as ampicillin, cefoxitin, cephalothin, amoxicillin y clavulanic acid, and chloramphenicol, suggesting that prepared foods sold on the street could represent a potential route of dissemination of resistant in the food chain. The profiles of multi-resistance and emerging resistance to antibiotics were identified, highlighting the presence of beta-lactam resistance genes blaTEM and carbapenemase resistance gene blaCMY. Considering their epidemiological importance is required further investigation using whole-genome sequencing techniques.
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    Evaluación de la resistencia antimicrobiana de Enterobacterias aisladas a partir de vegetales de la ciudad de Riobamba
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Maestría en Ciencia de los Alimentos, 2022-05) Barragán Fonseca, Gabriela Cecilia; Calero Cáceres, William Ricardo
    The monitoring of hygienic conditions throughout the food production chain aims to prevent the transmission of pathogenic microorganisms. The safety of raw vegetables and fruits is being affected by the presence of Enterobacteriaceae that possess complex mechanisms of antimicrobial resistance and virulence. In Riobamba (Ecuador) it was found that a high percentage of the vegetables shipped in the city's markets harbor Escherichia coli, Klebsiella spp. and Enterobacter spp., bacteria that show a considerable amount of multi-resistance patterns to different antibiotics. The antibiotic resistance by phenotypic evaluation detected high rates of resistance to the clinically-important beta-lacta antibiotic ampicillin and trimethoprim sulfamethoxazole. The molecular detection of antibiotic resistance genes detected de presence of beta-lactamases blaCTX-M and blaTEM, as well as carbapenemases blaCMY and blaSHV, that were considered emerging resistance mechanisms. On the other hand, the evaluation of virulence genes of diarrheagenic E. coli revealed the presence of enterohemorrhagic E. coli (EHEC) and enteroadherent E. coli (EAEC) pathotypes in some isolates. Although its finding is not numerous, the presence of this pathotypes is of concern due to the great frequency with which the EHEC variant appears in reports of foodborne diseases. Considering this result, the vegetables marketed in the city of Riobamba may constitute a potential route of dissemination of pathogenic bacteria and antimicrobial resistance through food chain.