Ciencia e Ingeniería en Alimentos y Biotecnología

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Subject: search.filters.subject.EDULCORANTES SINTÉTICOS

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    Determinación de edulcorantes por espectrofotometría visible en bebidas azucaradas consumidas en la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03) Salinas Lara, Anthony Daniel; Pérez Aldas, Lander Vinicio
    As sugar-sweetened beverages are considered a product of mass consumption, their components must be controlled, in this case synthetic sweeteners, which are food additives that must comply with regulations in their concentrations so that the food is not harmful to their consumers. The present investigation was oriented to determine the amount of synthetic sweeteners in the most consumed sugary drinks in the province of Tungurahua, such a selection took as a reference the research project called Tax Policy and the consumption of sugary drinks in Ecuador that was executed in the FCAUD In addition, a bibliographic comparison was made with the chromatographic technique to guarantee the reliability of the results obtained. The determination of the concentration of sweeteners in sugary drinks was carried out by means of spectrophotometry, supported by the research project of Castillo (2022), the results were 112.03 mg per liter for aspartame, 59.71 mg per liter for acesulfame and 85.83 mg per liter for sucralose. The experimental values did not present as much discrepancy with the chromatographic bibliographic values. Compliance with the CODEX STAN 192-1995 standard was verified since the concentrations of synthetic sweeteners in sugar-sweetened beverages presented values lower than those established as the maximum dose, which ensures that these beverages are effective and safe for human consumption.
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    Validación del método analítico por espectrofotometría uv-vis para la determinación de edulcorantes en bebidas azucaradas consumidos en la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03) Castillo Mayorga, Alison Abigail; Pérez Aldas, Lander Vinicio
    The present research focuses on the validation of the calibration curves of the UV Vis spectrophotometry analysis method for the detection of synthetic sweeteners of sucralose, aspartame and acesulfame K, the study is aimed at possible applications in sugary drinks. For the analysis, standard solutions were prepared with buffer and chromophore solutions in known concentrations. The study was carried out in a UV Vis Thermo Scientific Evolution 60S series spectrophotometer. The calibration curves were made with the proposed concentrations for each standard solution with which the value of R squared was determined, in all cases it was greater than 99 percent, in turn, the equations of calibration for each solution. A statistically significant linearity was determined for the process and a sensitivity of 0.0252; 0.0018 and 0.2332 for sucralose, aspartame and acesulfame K. The detection limit calculated for each standard solution corresponds to 0.0547, 0.0249 microgram per milliliter and 0.0459 milligram per milliliter for sucralose, aspartame, and acesulfame K, respectively; the calculated expanded uncertainty was between 7.44, 19.08 and 21.5 percent for sucralose, aspartame and acesulfame K, respectively. In turn, through a bibliographic review, it was possible to determine that HPLC chromatography is a method for detecting high precision synthetic sweeteners with very sensitive detection limits, however, spectrophotometry is also a good option for this type of analysis that presents a great sensitivity.