Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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Tema: search.filters.subject.BEBIDAS AZUCARADAS

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Mostrando 1 - 4 de 4
  • Miniatura
    Item type:Ítem,
    Cuantificación simultanea de conservantes y cafeína en bebidas azucaradas consumidas por los moradores de la provincia de Tungurahua, por espectroscopia ultravioleta
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03)
    Verdesoto Martínez, Solange Monserrath
    ;
    Pérez Aldas, Lander Vinicio
    Energy drinks such as Monster Energy and 220V, which contribute to increased energy, physical performance and a better mental state of the people who consume it, on the other hand, Coca-Cola light is a carbonated drink made up of sugar in the form of monosaccharides and added caffeine that stimulates the nervous system; In order to preserve the properties and nutritional value of the drink, preservatives such as sorbic acid and benzoic acid are used, recognized by the CODEX192 standard as additives that inhibit the formation of bacteria, molds and yeasts. The concentrations of both preservatives and caffeine in the mentioned beverages were determined, for which a method validated by UV-VIS spectrophotometry was used, the respective absorbances were measured and due to the matrix effect the addition of standard was used, where it was obtained that sorbic acid is present in the three drinks, but with a higher quantity in Monster since it has 46,778 mg-Kg, benzoic acid mostly in 220V 34,415 mg-kg compared to the other drinks, on the other hand, caffeine also It is part of the composition of the three samples, mostly in Monster with 274.276 mg-kg, followed by 220V with 251.017 mg-kg and finally Coca-Cola Light with 40.448 mg-kg. All concentrations are within the ranges established by the CODEX192 and NTE-INEN regulations, however, excessive consumption can cause health problems, therefore, it is recommended not to exceed the daily intake established based on your body weight.
  • Miniatura
    Item type:Ítem,
    Determinación de edulcorantes por espectrofotometría visible en bebidas azucaradas consumidas en la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03)
    Salinas Lara, Anthony Daniel
    ;
    Pérez Aldas, Lander Vinicio
    As sugar-sweetened beverages are considered a product of mass consumption, their components must be controlled, in this case synthetic sweeteners, which are food additives that must comply with regulations in their concentrations so that the food is not harmful to their consumers. The present investigation was oriented to determine the amount of synthetic sweeteners in the most consumed sugary drinks in the province of Tungurahua, such a selection took as a reference the research project called Tax Policy and the consumption of sugary drinks in Ecuador that was executed in the FCAUD In addition, a bibliographic comparison was made with the chromatographic technique to guarantee the reliability of the results obtained. The determination of the concentration of sweeteners in sugary drinks was carried out by means of spectrophotometry, supported by the research project of Castillo (2022), the results were 112.03 mg per liter for aspartame, 59.71 mg per liter for acesulfame and 85.83 mg per liter for sucralose. The experimental values did not present as much discrepancy with the chromatographic bibliographic values. Compliance with the CODEX STAN 192-1995 standard was verified since the concentrations of synthetic sweeteners in sugar-sweetened beverages presented values lower than those established as the maximum dose, which ensures that these beverages are effective and safe for human consumption.
  • Miniatura
    Item type:Ítem,
    Validación del método de cuantificación simultánea de conservantes y cafeína presentes en bebidas azucaradas por espectroscopía ultravioleta en la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03)
    Ojeda Armijos, Segundo Javier
    ;
    Pérez Aldas, Lander Vinicio
    Sorbic acid and benzoic acid are used as preservatives, in order to avoid the presence of microorganisms in sugary drinks. Instead, caffeine acts on the nervous system, which is why it is used as a stimulant. However, the excessive use of preservatives and caffeine in sugary drinks can cause health problems in consumers, so it is important to know the concentrations present in the samples of Coca Cola Light, 220V and Monster, which must be within the values established by the NTE INEN-CODEX 192, INEN 1101 and INEN 2411 standards. The validation of the spectrophotometric method was carried out in the LACONAL laboratory, using the 60S EVOLUTION equipment, implementing the standard addition method, preparation of calibration curves and application of analytical parameters such as precision, repeatability, reproducibility, accuracy, linear range, working range and sensitivity detailed in the Eurachem guide. The analytical parameters had the acceptance criteria, through the application of the Fisher and t-Student test, the percentage Absolute Error, the Relative Error and the Horwitz CV. Finally, the uncertainty of: repeatability and reproducibility, the verification of the spectrophotometric equipment, these uncertainty values allow the combined uncertainty calculation, which is used to obtain the expanded uncertainty where the maximum value was more less 2, 49 mg for ml. A maximum value of the percentage of expanded uncertainty of 5.77, was obtained, which is less than the established 30 percent.
  • Miniatura
    Item type:Ítem,
    Validación del método analítico por espectrofotometría uv-vis para la determinación de edulcorantes en bebidas azucaradas consumidos en la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03)
    Castillo Mayorga, Alison Abigail
    ;
    Pérez Aldas, Lander Vinicio
    The present research focuses on the validation of the calibration curves of the UV Vis spectrophotometry analysis method for the detection of synthetic sweeteners of sucralose, aspartame and acesulfame K, the study is aimed at possible applications in sugary drinks. For the analysis, standard solutions were prepared with buffer and chromophore solutions in known concentrations. The study was carried out in a UV Vis Thermo Scientific Evolution 60S series spectrophotometer. The calibration curves were made with the proposed concentrations for each standard solution with which the value of R squared was determined, in all cases it was greater than 99 percent, in turn, the equations of calibration for each solution. A statistically significant linearity was determined for the process and a sensitivity of 0.0252; 0.0018 and 0.2332 for sucralose, aspartame and acesulfame K. The detection limit calculated for each standard solution corresponds to 0.0547, 0.0249 microgram per milliliter and 0.0459 milligram per milliliter for sucralose, aspartame, and acesulfame K, respectively; the calculated expanded uncertainty was between 7.44, 19.08 and 21.5 percent for sucralose, aspartame and acesulfame K, respectively. In turn, through a bibliographic review, it was possible to determine that HPLC chromatography is a method for detecting high precision synthetic sweeteners with very sensitive detection limits, however, spectrophotometry is also a good option for this type of analysis that presents a great sensitivity.