Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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2024 - 20264

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Fecha de inicio: 2024Tema: search.filters.subject.ASTAXANTINA

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Mostrando 1 - 4 de 4
  • Miniatura
    Item type:Ítem,
    Determinación de las condiciones óptimas de cultivo de Haematococcus pluvialis para la producción de biomasa y astaxantina
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2026-01)
    Esquivel Galvez, Kevin Wladimir
    ;
    García Solís, Mario Daniel
    This study evaluated the optimization of culture conditions for Haematococcus pluvialis to maximize biomass and astaxanthin production, a pigment of high biotechnological value. The main objective was to determine the optimal cultivation conditions by comparing three media (BBM, Fuerza Verde, and Bonanza) under two aeration rates (250 and 1000 mL per min). The methodology included a factorial design, monitoring of growth kinetics through cell counting, biomass quantification by dry weight, induction of carotenogenesis using light and salt stress, and pigment extraction assisted by ultrasound. The results showed that Bonanza achieved the highest cell densities and productivities, confirming its non-limiting nutrient profile. Fuerza Verde exhibited intermediate values, while BBM displayed moderate growth with a final decline associated with nutrient limitation. ANOVA revealed a significant effect of the culture medium and the medium per aeration interaction on cell density. Regarding astaxanthin, only BBM showed the characteristic absorption peak at 482 nm and measurable pigment concentrations, whereas Fuerza Verde and Bonanza maintained chlorophyll-dominated profiles linked to their higher NPK availability. Overall, enriched media favored biomass accumulation, whereas nutrient-limited conditions, such as those in BBM, promoted carotenoid induction. This work provides comparative evidence on the combined influence of nutrients and aeration on the physiology of Haematococcus pluvialis, contributing to the development of more efficient cultivation strategies for biotechnological applications.
  • Miniatura
    Item type:Ítem,
    Evaluación de la actividad antioxidante, carotenoides totales, rendimiento global y perfil de extractos obtenidos a partir de cáscaras de camarón mediante propano subcrítico y Soxhlet
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-07)
    Naranjo Carrillo Victor Mateo
    ;
    Cadena Carrera Santiago Esmiro
    Ecuador is one of the world's leading producers of shrimp (Litopenaeus vannamei), generating approximately 1.2 million tons annually, of which around 40 percent corresponds to by-products such as shells and heads. This study aimed to valorize these residues through the extraction of bioactive compounds, particularly astaxanthin, using two methods: Soxhlet extraction, considered a standard procedure for bioactive compound extraction, and subcritical propane extraction, an environmentally friendly alternative. Global yields were determined, antioxidant capacity was evaluated using the DPPH assay, total carotenoids were estimated by spectrophotometry, and qualitative composition was analyzed by thin-layer chromatography (TLC). The results showed that Soxhlet extraction yielded 2,55 percent, significantly higher than the 0,15 percent obtained with subcritical propane. Regarding total carotenoid concentration, Soxhlet produced 8,55 micrograms per milliliter, compared to 3,80 micrograms per milliliter for subcritical propane. However, the extracts obtained with subcritical propane exhibited greater antioxidant capacity, reaching 68,48 percent, compared to 51,85 percent for Soxhlet. Additionally, better migration patterns were observed in TLC for the subcritical propane extracts, suggesting higher purity, likely due to the solvent’s selectivity.
  • Miniatura
    Item type:Ítem,
    Obtención de astaxantina a partir de exoesqueletos de cangrejo rojo (Ucides occidentalis)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Alimentos, 2025-02)
    Chiliquinga Angulo, Joel Alexander
    ;
    Cadena Carrera, Santiago Esmiro
    Astaxanthin is a carotenoid with outstanding biological activity, and its antioxidant capacity surpasses those of other carotenoids. Since the use of synthetic astaxanthin is restricted, there has been growing interest in obtaining this compound from natural sources. Astaxanthin is responsible for the characteristic reddish hue of the red crab (Ucides occidentalis). In Ecuador, approximately 40 million red crabs are caught annually for consumption, which generates a sanitation problem due to the large amounts of resulting by-products, as 70 to 80 percent corresponds to the inedible fraction of crustaceans. Considering this, the present study evaluated the overall extraction yield and the amount of astaxanthin present in each extract using extraction techniques such as Soxhlet and maceration, considering the extraction time. The overall extraction yield and astaxanthin content were evaluated by gravimetry and spectrophotometry, respectively. The highest overall yield was obtained using the Soxhlet technique at 210 min (0.120 percent), and the highest astaxanthin content was obtained using the Soxhlet technique at 180 min (332.132 microgram per gram). The antioxidant capacity of the extract with the highest astaxanthin content was evaluated by the DPPH method, where 48.779 percent inhibition equivalent to 250.620 micromoles of Trolox per liter was obtained. Therefore, the present investigation demonstrated the production of astaxanthin, evaluating its yield as a function of the extraction time with ethanol and its antioxidant capacity.
  • Miniatura
    Item type:Ítem,
    Optimización del proceso de extracción de compuestos bioactivos mediante superficie de respuesta a partir de residuos de camarón
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Alimentos, 2024-08)
    Villafuerte Herrera, Ronaldo Julián
    ;
    Cadena Carrera, Santiago Esmiro
    Astaxanthin is a carotenoid with significant biological activity, even greater antioxidant activity than other carotenoids. Synthetic astaxanthin has usage restrictions, which has led to interest in obtaining it from natural sources. Astaxanthin is responsible for the pinkish color of shrimp, and the cultivation of shrimp has increased globally, along with its negative effects, primarily the generation of waste that constitutes approximately 25 to 60 percent by weight. Considering this, the present study evaluated the extraction of astaxanthin using Soxhlet and modeled the extraction kinetics from shrimp shells (Litopenaeus vannamei). The raw material was characterized by proximal analysis, particle size, and shape. The overall extraction yield and astaxanthin content were evaluated by gravimetry and spectrophotometry, respectively. The highest overall yield was obtained with the ethanol plus water solvent (3.75 percent), and the highest astaxanthin concentration was for the ethanol and methanol solvents (292.2 and 296.0 micrograms per gram, respectively). The model that best fit to describe the extraction kinetics was the Peleg model, with a determination coefficient between 0.93 and 0.98. This research demonstrates the potential recovery of astaxanthin from shrimp waste using ethanol.