Maestría en Laboratorio Clínico Mención Microbiología Clínica
Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/42733
Browse
2 results
Search Results
Item COINCIDENCIAS DEL DIAGNÓSTICO DEL GÉNERO STREPTOCOCCUS ENTRE MÉTODO MANUAL Y EL MÉTODO AUTOMATIZADO EN EL LABORATORIO AUTOMATIZADO ZAMORA(Universidad Técnica de Ambato / Facultad de Ciencias de Salud / Centro de Posgrados, 2024-10-01) Zamora Sánchez, María Cristina; Guangasig Toapanta, Víctor HernánThe genus Streptococcus sp. They are gram positive cocci; these bacteria grow in chains or pairs which are oxidase and catalase negative. Among the groups that cause disease in humans are the most frequent such as Streptococcus pyogenes (group A) produces tonsillitis and impetigo, Streptococcus pneumoniae is the cause. Of the majority of pneumonias acquired in the community, Streptococcus viridans and its groups are the cause of dental abscesses, within the subgenus is Streptococcus mutas the main agent that causes dental cavities and without forgetting that it causes endocarditis is a common pathogen that causes severe and potentially complicated infections with the possibility of generating serious sequelae, hence the importance of rapid, accurate and timely identification. In this context, it is essential to evaluate the available diagnostic methodologies to determine their relative effectiveness. In particular, the performance of the manual method should be compared with the Vitek 2 Compact automated system, in order to evaluate the concordance between both approaches in the identification of Streptococcus sp. The objective of this study is To evaluate the agreement of the diagnosis of Streptococcus sp. between the manual method and the automated method in the Zamora Automated Clinical Laboratory in the period July and May 2023-2024. The methodology used for this study was Quantitative, non-experimental, observational, descriptive study with verification of coincidences in diagnostic tests for the identification of 100 culture samples with growth for Streptococcus sp. in two laboratories, verification of coincidences in diagnostic tests for identification in 100 culture samples with growth for Streptococcus sp. in two laboratories, using conventional and automated methods (Vitek 2 Compact). To obtain results, tests were carried out to Differentiation tests were performed, obtaining 100% negative catalase, 100% alpha hemolysis, 100% resistant bacitracin, 94% resistant optochin. The identification of pathogens of the genus Streptococcus sp. was carried out using two different methods: one manual and the other automated. The manual method, revealed the following proportions: 94% corresponded to Streptococcus of the viridans group, 6% to S. pneumoniae, and 0% to S. pyogenes. On the other hand, the automated method provide results with 89% of S. viridans, 6% of S. pneumoniae and 0% of S. pyogenes. These findings show a slight discrepancy in the proportion of S. viridans identified by both methods, while the identities of S. pneumoniae and S. pyogenes remained constant between the two analytical approaches. The kappa index determined a concordance of 1 (almost perfect) according to the table between both methods for the identification of S. pneumoniae and 0.635 (considerable for identifying S. viridans. Based on these findings, a proposal was designed for the improvement of diagnostic skills according to the results obtained, the identification of the Streptococcus sp. by the manual method compared to the automated methodology (Vitek 2 Compact), presents high concordance, especially with S. pneumoniae. Strategies must be reviewed and implemented to improve the identification of this pathogen in laboratories how is the implementation of the automated equipment since it has a better identification in the subgenera of the viridans group which are of clinical interest in the dental areaItem CARACTERIZACIÓN MICROBIOLÓGICA DE AGENTES CAUSANTES DE ONICOMICOSIS EN TRABAJADORES DEL MERCADO MAYORISTA AMBATO(Universidad Técnica de Ambato / Facultad de Ciencias de Salud / Centro de Posgrados, 2024-09-04) Villacrés Taco, Lorena Viviana; Salazar Garcés, Dolores KrupskayaOnychomycosis has become a public health problem in Ecuador, generating a negative and significant impact on people's quality of life. The present study aimed to microbiologically characterize the agents causing onychomycosis in samples of nail lesions of workers at the Ambato Wholesale Market in the period October 2023 - May 2024. In this research, a non-experimental cross-sectional, descriptive and observational study was carried out in which a quantitative and qualitative approach was applied due to the identification of yeast-like mycotic agents and their sensitivity and resistance profile to antifungals in nail lesions of workers. From 212 participants undergoing the research, of which 111 met the requirements and toenail samples were taken, the following results were obtained: a total of 33 positive samples were identified where Candida spp. representing around 69.7%, Trichsoporum rubrum with 12.1%, Epidermophyton floccosum in 9.1% and Aspergillus with 9.1%. It is important to take into account that the Mycosel Agar culture medium was used. In the same way, Candida albicans was identified by culture in CHROMagar in 87%, with Candida parasilopsis 13%. In the samples analyzed, the main agents were Candida species, with a predominance of C. albicans followed by C. parapsilosis and sensitivity to Fluconazole was identified in 90% for C, albicans while in C. parapsilopsis this response was 33.33% and for Itraconazole it was observed that C. parapsiolpsis was sensitive in 100% while for C. albicans the sensitivity was 95%. In response to this, a diagnostic protocol for onychomycosis was developed, with improvement actions in the pre-analytical and analytical phase applicable in the Zamora Ambato Clinical and Bacteriological Laboratory.