Carrera de Biotecnología
Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34800
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Item Caracterización de la producción de metabolitos primarios y secundarios por Scenedesmus sp. nativa del Ecuador(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Paredes Paredes, Jennifer Katheryn; García Solís, Mario DanielCurrently, global problems have arisen, such as the limited availability of food and population growth, causing the depletion of natural resources, emissions of greenhouse gases and nitrous oxide from fertilizers and pesticides, methane production by ruminants, and deforestation. At the same time, the need to feed the growing population has driven the development of techniques to obtain microalgae supplements, since they produce active compounds and metabolites such as carbohydrates, proteins, pigments, vitamins and amino acids. For this reason, this research seeks to evaluate the production of primary and secondary metabolites by the microalga Scenedesmus sp. native to Ecuador, quantifying its capacity to produce proteins, carbohydrates and pigments under different culture conditions. Supplements to the BBM medium with different concentrations of sodium nitrate (0, 1.25, 2.5 and 5 grams per liter) and carbon sources (carbon dioxide, ethylene glycol, sodium acetate) were used. These conditions were combined to obtain 12 treatments. The culture scenario was 21 degrees Celsius, a light-dark cycle of 16 and 8 hours, and constant aeration of 200 milliliters per minute with 0.04 percent volume-volume carbon dioxide. The results showed that the best treatment was the mixotrophic culture with ethylene glycol and high concentration of sodium nitrate, producing the highest amount of proteins, carbohydrates and pigments (chlorophyll and carotenoids). It was also determined that the growth kinetics of Scenedesmus sp. varies according to the concentration of sodium nitrate in the medium.Item Evaluación de la actividad antifúngica de un microencapsulado rico en polifenoles del residuo de caña de azúcar (Saccharum officinarum L) frente a Botrytis cinerea(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Abril Díaz, Viviana Gabriela; López Hernández, Orestes DaríoCurrently, biotechnology is looking for alternatives to replace commercial fungicides in the control of pathogens such as Botrytis cinerea, responsible for approximately 10 percent of annual crop losses. Research on the use of plant residues, whose metabolites fulfill this purpose without causing harm to the environment or humans, have emerged as central areas of study. Such is the case of sugarcane bagasse, rich in polyphenols with antifungal activity, being capable of being used in the future within the fields as a pest control agent. To obtain the extract rich in polyphenols, the organic solvent extraction method was used using ethanol at 30, 50 and 70 percent evaluated at 30, 45 and 60 minutes in a 1:20 plant material:solvent ratio. After this, the Folin test was performed to measure the concentration of total polyphenols, the best treatment being 50 percent at 30 minutes for 63 g per GAE. Next, microencapsulation of the concentrate was carried out at a 20:80 ratio using maltodextrin, using the spray drying technique, and its efficiency was evaluated using FTIR. Finally, the in vitro antifungal activity was evaluated at 10, 30 and 50percent microencapsulation as an antibiogram, obtaining a higher percentage of inhibition at 50 percent microencapsulation than the total average of the 8 days of the trial.Item Evaluación del efecto antifúngico del microencapsulado de aceite esencial extraído de Schinnus molle sobre hongos fitopatógenos de interés agrícola(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Montachana Ibarvo, Josafat Alexander; López Hernández, Orestes DaríoThe molle is a tree of Andean origin that throughout history has been used for medicinal, culinary, textile purposes, among others, also demonstrating strong biological activity in the agricultural area for the control of pests in insects, bacteria, and fungi since it has antimicrobial properties. This is why its essential oil may be important for the development of a fungicide of biological and plant origin, which serves to control several agricultural diseases caused by phytopathogenic fungi. In the present research project, the essential oil of molle was extracted by the steam drag method, obtaining a yield of 2.8 percent, in addition to the conservation of the essential oil, the microencapsulation method by spray drying was used using of a mixture of polymers with maltodextrin and gum arabic as microencapsulating agents, in which the microencapsulation performance was qualitatively verified by means of Fourier transform spectroscopy (FTIR). Finally, with the Kirby Bauer method, it was verified in vitro that with a concentration of 100 milligrams per milliliters of microencapsulated molle essential oil there is sensitivity to Botrytis cinerea, Cladosporium spp and Fusarium spp.Item Caracterización de la producción de metabolitos secundarios por microorganismos fotosintéticos(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Lalaleo Camino, Christian Josué; García Solís, Mario DanielGlobal population growth has driven demand for food resources, causing a negative environmental impact through the expansion of agriculture and livestock farming. In response, photosynthetic microorganisms have been highlighted as an alternative source of bioactive compounds, such as proteins, carbohydrates, and pigments. This project evaluated the impact of carbon and nitrogen on the growth kinetics, production of proteins, carbohydrates, and pigments of the CLMR1 isolate, as well as its influence on cell morphology. Autotrophic and mixotrophic cultures were carried out, using carbon dioxide with two organic carbon sources (ethylene glycol and sodium acetate) and different concentrations of sodium nitrate (0, 0.625, 1.25 and 2.5) grams per liter. The most outstanding results were observed in the culture with 2.5 grams per liter of sodium nitrate and ethylene glycol, achieving the highest cell concentration (21,000,000 cells per milliliter), maximum amount of carbohydrates (1015.78 micrograms per milliliter) and the highest synthesis of chlorophyll. and carotenoids (0.274 and 0.207 micrograms per milliliter). Additionally, treatment with 2.5 grams per liter of sodium nitrate and sodium acetate produced the highest protein concentration (2.31 micrograms per milliliter). However, cultures without sodium nitrate showed the lowest synthesis of each metabolite and the minimum biomass production (200,000 cells per milliliter). Morphological changes were evident in the cells, highlighting the importance of optimal culture conditions for the synthesis of metabolites and their application in the sustainable production of high-value biomolecules.Item Evaluación de la actividad antioxidante y antiinflamatoria de las flores de Spartium junceum(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03) Solis Mayorga, Erick Xavier; Pérez Salinas, Ruth NarcisaIn rural areas of developing countries, traditional medicine is used as the only treatment for illnesses, since it is difficult for them to go to a hospital or because they cannot afford to buy the medicines. Traditional medicine has been found to have an endless number of benefits such as antivirals, antioxidants, anti-inflammatory, anticancer, etc. Therefore, the present research aimed to evaluate the antioxidant and anti-inflammatory activity of S. junceum flowers. Hydroalcoholic extracts were obtained by Soxhlet extraction and maceration with 96 percent and 70 percent ethanol, the extract by Soxhlet with 70 percent ethanol had the highest yield of 14.01 percent with a standard deviation of 3.64. The qualitative determination of secondary metabolites was developed by phytochemical assays and metabolites such as alkaloids, saponins, phenols, tannins, flavonoids and quinones were identified in different concentrations; and to verify the presence of these, infrared spectrophotometry was also performed. The antioxidant capacity was performed with the DPPH technique, where the Soxhlet extract with 70 percent ethanol obtained the highest antioxidant activity of 76.72 percent with a standard deviation of 0.01. Finally, the extracts were evaluated in the anti-inflammatory activity by inhibition of egg albumin denaturation, the highest inhibition of 76.55 percent with a standard deviation 0.05 corresponded to the Soxhlet extraction with 96 percent ethanol.Item Evaluación de la actividad antioxidante y antimicrobiana de la hoja y fruto del mortiño (Vaccinium floribundum Kunth) frente a bacterias patógenas (Bacillus cereus, Staphylococcus aureus, Escherichia coli y Listeria monocytogenes)(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2022-09) Guamán Poaquiza, Edith Johanna; Tubón Usca, Irvin RicardoThe resistance of multiresistant bacteria to antibiotics is a global health problem, which has generated various infectious diseases transmitted by contaminated food. Therefore, the OMS promotes innovation and research of new sources that counteract this problem. For this reason, this research project aimed to evaluate the antioxidant activity of leaves and fruits of V. floribundum Kunth and the antimicrobial activity against pathogenic bacteria. First, ethanolic extracts were obtained, resulting in yields of 11,189 and 41,163 percent of leaf and fruit extract, respectively. The qualitative determination of secondary metabolites was developed through phytochemical assays and metabolites were obtained such as: flavonoids and tannins in the leaf and flavonoids, tannins, phenols and diterpenes in the fruit. The antioxidant capacity with the DPPH technique was performed, where 86,422 and 27,930 percent of the leaf and fruit were obtained. Finally, the agar diffusion method was used to evaluate the antimicrobial activity. The inhibition halos in the leaf extract were: L. monocytogenes (20,667 mm), S. aureus (17,833 mm), B. cereus (18,500 mm) and E. coli (13,667 mm) and in the fruit extract: L. monocytogenes (14,000 mm), S. aureus (11,667 mm), B. cereus (11,500 mm) and E. coli (9,833 mm). The best percentage of inhibition was of S. aureus with 89 and 38,4 percent of the leaf and fruit with Vancomycin and with Gentamicin L. monocytogenes obtained 68,3 and 35 percent of the extract of the leaf and fruit.Item Actividad antiviral de aceites esenciales de plantas(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2022-09) Barona Endara, Joselyn Michelle; Arancibia Soria, Mirari YosuneEssential oils are highly volatile aromatic substances that are extracted from plant material from different parts of plants. It has been shown in various bibliographic sources that these compounds can inhibit different types of viruses due to their varied composition of bioactive substances and represents a potential source of active principles for its use in the pharmaceutical industry. However, the availability of summaries with synthesized information on a variety of these is limited, which leads to a general lack of knowledge of their benefits. The objective of this study is to identify the main essential oils that have antiviral activity. With this aim, the research question is the following: ¿Which essential oils exhibit antiviral activity according to the literature reviewed in bibliographic databases? This is answered through an exhaustive investigation of quantitative data of in vitro antiviral activity of 42 types of plants. The data found are classified according to the percentage of inhibition in the categories of high (total inhibition), high, moderate, low, no activity and no activity with enhancement of viral activity. These results indicate varied antiviral activity, of the essential oils investigated, several of them have a total inhibition capacity of certain viruses, while others, despite not eliminating them, have a high antiviral activity and, on the other hand, certain essential oils demonstrate a viral potentiating ability. Taking this into account, it is recommended to use the tabulated data for future research to verify the efficacy of these substances.