Carrera de Biotecnología

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    Determinación de componentes fenólicos y antioxidantes en harina de papa china (Colocasia esculenta) y zanahoria blanca (Arracacia xanthorrhiza)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03) Silva Aldaz, Melanie Carolina; Pérez Aldas, Lander Vinicio
    Currently, a public health problem is the simultaneous presence of malnutrition and overweight, 11 percent of children under 5 years of age in Latin America and the Caribbean present serious malnutrition problems and 7 percent present overweight problems, in Ecuador it has been evidenced the tendency to suffer from malnutrition and overweight, This is directly related to the current diets, which are rich in fats, sugar and salt, therefore, this work focused on determining and highlighting the phenolic compounds present in Andean crops such as the Chinese Potato and White Carrot and determining their antioxidant capacity. Flour is one of the main products in the food area, therefore, the phenolic compounds present in flour of Chinese Potato and White Carrot were determined by HPLC UV VIS analysis and the antioxidant capacity of these food matrices was determined by DPPH assay, where malonic acid, protocatechuic acid, hexoxide ferulic acid, gallic acid, 3- O-caffeoylquinic acid, rutin, 5-O-caffeoylquinic acid, quinol, quinic acid, p-coumaric acid, p-coumaric acid, p-coumaric acid, p-coumaric acid, p-coumaric acid and p-coumaric acid were identified, p-coumaric acid, chlorogenic acid and caffeic acid, which are considered nutraceuticals of great importance as they have great health benefits, especially for the prevention of chronic diseases such as diabetes II, heart disease, obesity and metabolic syndrome, thanks to their antioxidant properties, obtaining an inhibition percentage of DPPH radicals of 24.29 percent for the white carrot flour and 18.61 percent for the Chinese potato flour.
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    Determinación del perfil lipídico en harinas de chocho (Lupinus mutabillis sweet) y melloco blanco (Ullucus tuberosus) para establecer el contenido de ácidos grasos saturados e insaturados
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03) Sánchez Paredes, Daniela Alejandra; Pérez Salinas, Ruth Narcisa
    Flour is one of the central products for the production of functional foods, in Ecuador there are several crops with quality nutritional content, however, they are undervalued and no studies have been conducted to exploit their benefits. On the other hand, lipids are a fundamental part for the correct metabolic functioning, however, their excessive consumption is not recommended for health. For this reason, the present study evaluates the lipid profile of chocho and white melloco flour, in order to determine if the fat content in these matrices will allow the formation of products that provide health benefits to the Ecuadorian population. Fatty acids were determined with FID gas chromatography, in which 7 fatty acids were identified in chocho; palmitic acid (10.38 percent), oleic acid (51.92 percent), linoleic acid (40.43 percent), linolenic acid (2.45 percent), stearic acid (3. 17 percent), behenic acid (0.92 percent), arachidic acid (0.73 percent); while, the lipid profile of melloco recognized 4 fatty acids; palmitic acid (15.43 percent), oleic acid (5.92 percent), linoleic acid (71.94 percent), linolenic acid (6.71 percent). In addition, nutritional quality indices omega 6: omega 3 (12.42; 10.72), PUFA:SFA (2.16; 5.08) and atherogenicity (0.12;0.18) were performed on chocho and melloco blanco, respectively.
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    Determinación del perfil lipídico en harinas de papa china (Colocasia esculenta) y zanahoria blanca (Arracacia xanthorrhiza) para establecer el contenido de ácidos grasos saturados e insaturados
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03) Moreta Freire, Joselyn Beatriz; Pérez Aldas, Lander Vinicio
    Ecuador is one of the 17 megadiverse countries in Andean tuber and root crops that over time have been industrialized in the production of flour. Currently, the population includes vegetable flours in their daily diet, which have high nutritional value, dietary fiber, starch, minerals, vitamins, carbohydrates, and lipids necessary to provide energy to the body. It is worth mentioning that high consumption of saturated fats can lead to cardiovascular diseases. Therefore, the purpose of this research was to determine the lipid profile of chinese potato (Colocasia esculenta) and white carrot (Arracacia xanthorrhiza) flours to establish saturated and unsaturated fatty acids, in order to identify the nutritional quality of these flours and guarantee a healthy diet. The fat component in Chinese potato and white carrot flour was extracted by Soxhlet equipment with results of 0.40 plus minus 0.04 percent and 0.84 plus minus 0.08 percent, respectively. The lipid profile was determined by gas chromatography with flame ionization detector, identifying the main fatty acids: palmitic acid (30.83 percent and 18.68 percent); oleic acid (28.53 percent and 15.97 percent) and linoleic acid (40.64 percent and 65.35 percent). Quality indexes were also established that indicated that they were good flours for inclusion in the daily diet.
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    Determinación de componentes fenólicos y antioxidantes en harina de camote morado (Ipomeas batatas) y mashua (Tropaeolum tuberosum)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03) Chamba Maza, Jonnatan Franklin; Pérez Aldas, Lander Vinicio
    The lack of awareness about the consumption of functional and nutritious foods has generated multiple problems in health, being considered a negative factor that has been increasing over the years. For this reason and with the purpose of providing a contribution to society around health and human nutrition, this research project aimed to provide relevant information on the Andean crops of Ecuador, such as sweet potato and mashua, toexpose its composition in terms of its phenolic content and antioxidant capacity. This information can be exploited in a way that supports the development of nutritional foods that allow achieving a better quality of life by reducing nutritional health problems. First, the HPLCUV Visibletechnique was performed on flour samples for the identification of phenolic compounds using gallic acid as standard, determining compounds such as p-coumaric acid, cyanidin, caffeic acid and derivatives, tyrosol glycoside and catechin. The antioxidant capacity was determined by the DPPH technique, obtaining a percentage of 60.976 percent for sweet potato and 69.974 percent for mashua, which are considered significant, finding the mashua flour with a higher antioxidant capacity.