Tesis Medicina Veterinaria y Zootecnia

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/872

Browse

Filters

20172

Settings

Author: search.filters.author.Solís Villacrés, Ricardo David

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Efecto de la adición de bacillus spp. en ensilaje de maíz (zea mays) sobre la cinética de degradación ruminal in situ y fermentación ruminal in vitro.
    (2017) Solís Villacrés, Ricardo David; Barros Rodríguez, Marcos
    The effect of the addition of Bacillus spp. in maize (Zea mays) stubble silage was evaluated on the in situ ruminal degradation kinetics and ruminal fermentation in vitro according to a completely randomized design with four dose treatments of Bacillus spp. (0, 1.5, 3, 4.5 ml/Kg DM). Determined the content of Dry Matter (DM), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (FDA) and the Effective Degradation (ED) of DM. In vitro digestibility consisted of incubating the samples with ruminal fluid for 48 h, in situ degradation of MS was performed in 6 male castrated cattle, weighing approximately 450 kg of mestizo breed, with a cannula fistula in the rumen (Bar Diamond, Parma, Idaho, USA). Maintained in zinc roof pens and cement floor, the animals were fed with a diet based on alfalfa fodder, the incubation times were 0, 3, 6, 12, 24, 36, 48, 72 and 96 h. The data obtained were adjusted to the model of Orskov & McDonald (1979) to establish the soluble (a), insoluble but potentially degradable fractions (b), degradation rates in % per hour (c), passage rates (k) 0.02, 0.05 and 0.08 (k /% h) and effective degradability (ED). Regarding the degradability of DM, the treatments T3 and T4 showed higher values in the insoluble but potentially degradable fraction (b) (45% and 44%, respectively). In relation to ruminal degradation of OM, soluble fraction (a) was higher (P = <.0001) in T1 (37%), T2 (39%) and T4 (38%) in relation to T3, the Insoluble fraction but potentially degradable (b) was higher (P = <.0001) in T3 (45%) and T4 (44%). The effective degradation with different passage rates (k) was greater (P> 0.05) in T2 and T4 than in the other treatments. The in vitro digestibility of DM and OM did not show differences (P = 0.1372 and P = 0.1169, respectively) between treatments. The ruminal pH in the 4 and 12 hours decreased in treatments T1, T2, T3 compared to T4 (7.11 and 7.36 respectively) post-incubation. Ammonia Nitrogen (NH3) presented statistical differences between treatments at hours 2, 4 and 8 post-incubation sampling, obtaining the highest (P <0.05) NH3 the experiments incubated with the silage treated with the highest dose of Bacillus spp. (T4). In the production of VFAs, propionic acid is higher in treatments T3 and T4 in hours (2, 4) and (2, 4 and 8) respectively.
  • No Thumbnail Available
    Item
    Efecto de la adición de Bacillus spp. en ensilaje de maíz (Zea mays) sobre la cinética de degradación ruminal in situ y fermentación ruminal in vitro.
    (2017) Solís Villacrés, Ricardo David; Barros Rodríguez, Marcos
    The effect of the addition of Bacillus spp. in maize (Zea mays) stubble silage was evaluated on the in situ ruminal degradation kinetics and ruminal fermentation in vitro according to a completely randomized design with four dose treatments of Bacillus spp. (0, 1.5, 3, 4.5 ml/Kg DM). Determined the content of Dry Matter (DM), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (FDA) and the Effective Degradation (ED) of DM. In vitro digestibility consisted of incubating the samples with ruminal fluid for 48 h, in situ degradation of MS was performed in 6 male castrated cattle, weighing approximately 450 kg of mestizo breed, with a cannula fistula in the rumen (Bar Diamond, Parma, Idaho, USA). Maintained in zinc roof pens and cement floor, the animals were fed with a diet based on alfalfa fodder, the incubation times were 0, 3, 6, 12, 24, 36, 48, 72 and 96 h. The data obtained were adjusted to the model of Orskov & McDonald (1979) to establish the soluble (a), insoluble but potentially degradable fractions (b), degradation rates in % per hour (c), passage rates (k) 0.02, 0.05 and 0.08 (k /% h) and effective degradability (ED). Regarding the degradability of DM, the treatments T3 and T4 showed higher values in the insoluble but potentially degradable fraction (b) (45% and 44%, respectively). In relation to ruminal degradation of OM, soluble fraction (a) was higher (P = <.0001) in T1 (37%), T2 (39%) and T4 (38%) in relation to T3, the Insoluble fraction but potentially degradable (b) was higher (P = <.0001) in T3 (45%) and T4 (44%). The effective degradation with different passage rates (k) was greater (P> 0.05) in T2 and T4 than in the other treatments. The in vitro digestibility of DM and OM did not show differences (P = 0.1372 and P = 0.1169, respectively) between treatments. The ruminal pH in the 4 and 12 hours decreased in treatments T1, T2, T3 compared to T4 (7.11 and 7.36 respectively) post-incubation. Ammonia Nitrogen (NH3) presented statistical differences between treatments at hours 2, 4 and 8 post-incubation sampling, obtaining the highest (P <0.05) NH3 the experiments incubated with the silage treated with the highest dose of Bacillus spp. (T4). In the production of VFAs, propionic acid is higher in treatments T3 and T4 in hours (2, 4) and (2, 4 and 8) respectively.