Tesis Medicina Veterinaria y Zootecnia

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Author: search.filters.author.Núñez Alverca, Karla Alejandra

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    Identificación de parásitos con diferentes métodos coprológicos en muestras de reptiles en el Vivarium de Quito
    (2021-08) Núñez Alverca, Karla Alejandra; Almeida Secaira, Roberto Ismael
    The current study has the objective to identify gastrointestinal parasites through three coprological methods in reptile fece samples of Vivarium Quito. 118 reptile samples were processed and analyzed; which 81 belong to 17 suborder Ofidia species, 23 samples belong to 7 different order Chelonia species and 14 samples belong to 5 suborder Saurus species. From this sample analysis, 88 (74.6%) samples result positive to any kind of parasite. For sampling, physician restraint were made according to the kind of reptil; it was used hooks or herpetological tubes to manipulate snakes, in case of poisonous specimens, they were managed by authorized staff, turtles and lizards were contained through manual restraint, turtles from the base of the tail or body and lizards avoiding his tail restraint, holding its paws. The samples were recolected with tongue depressors, kept in ziploc bags and kept refrigerated, then they were tagged with the scientific name of the animal, number of alive, area where are located in Vivarium and date of collection, transportation was made through of a cooler with cooling gels at a temperature of 4°C. The direct coprological, float and sedimentation methods were applied in each sample taken. As a result, 40 different kind of parasites were identified, 6 genera of Protozoan, 13 of Nematodes, 12 of Trematodes, 5 of Cestodes, 2 of Acantocephalus, 1 of Mites and 1 of Pentastomide type. From these results, the most frequently parasites were Blastocystis spp Protozoan (25%), nematodes within order of pinworms (23.9% egg) (11.4% adult), Balantidium sp (11.4%), Metamonádidos (10.2%), Strongyloides (9.1%), Kalicephalus sp (8%), Nyctotherus spp, Entamoeba sp., Ophionyssus natricis y Rhabdias spp these four kind of parasites with 6.8%. All identified genres were characterized morphologically according to their classification, presentation or status, shape, colour, size, and the coprological method which were identified. Finally, it was resulted that order Chelonia (82.6%) have the most frequency of parasites, then Squamata (72.6%), but separately Saurus has 100% of parasitism and Ofidia species 67.9%. In conclusion, there is a great variety of gastrointestinal parasites in reptiles of the study and it is priority to have a preventive deworming plan for avoiding being prone to future pathologies in these animals.
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    Identificación de parásitos con diferentes métodos coprológicos en muestras de reptiles en el Vivarium de Quito
    (2021-06) Núñez Alverca, Karla Alejandra; Almeida Secaira, Roberto Ismael
    The current study has the objective to identify gastrointestinal parasites through three coprological methods in reptile fece samples of Vivarium Quito. 118 reptile samples were processed and analyzed; which 81 belong to 17 suborder Ofidia species, 23 samples belong to 7 different order Chelonia species and 14 samples belong to 5 suborder Saurus species. From this sample analysis, 88 (74.6%) samples result positive to any kind of parasite. For sampling, physician restraint were made according to the kind of reptil; it was used hooks or herpetological tubes to manipulate snakes, in case of poisonous specimens, they were managed by authorized staff, turtles and lizards were contained through manual restraint, turtles from the base of the tail or body and lizards avoiding his tail restraint, holding its paws. The samples were recolected with tongue depressors, kept in ziploc bags and kept refrigerated, then they were tagged with the scientific name of the animal, number of alive, area where are located in Vivarium and date of collection, transportation was made through of a cooler with cooling gels at a temperature of 4°C. The direct coprological, float and sedimentation methods were applied in each sample taken. As a result, 40 different kind of parasites were identified, 6 genera of Protozoan, 13 of Nematodes, 12 of Trematodes, 5 of Cestodes, 2 of Acantocephalus, 1 of Mites and 1 of Pentastomide type. From these results, the most frequently parasites were Blastocystis spp Protozoan (25%), nematodes within order of pinworms (23.9% egg) (11.4% adult), Balantidium sp (11.4%), Metamonádidos (10.2%), Strongyloides (9.1%), Kalicephalus sp (8%), Nyctotherus spp, Entamoeba sp., Ophionyssus natricis y Rhabdias spp these four kind of parasites with 6.8%. All identified genres were characterized morphologically according to their classification, presentation or status, shape, colour, size, and the coprological method which were identified. Finally, it was resulted that order Chelonia (82.6%) have the most frequency of parasites, then Squamata (72.6%), but separately Saurus has 100% of parasitism and Ofidia species 67.9%. In conclusion, there is a great variety of gastrointestinal parasites in reptiles of the study and it is priority to have a preventive deworming plan for avoiding being prone to future pathologies in these animals.