Tesis Agronomía

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    Organogénesis directa de Solanum tuberosum L. Var. Superchola utilizando yemas brotadas de tubérculos
    (2023-03) Bonilla Vargas, Nicole Melanie; Leiva Mora, Michel
    The present study was developed in order to establish a method of micropropagation via direct organogenesis of plants of S. tuberosum L. Var. Superchola from bud sprouting induction, establishment, multiplication, rooting (in vitro) and subsequent ex vitro acclimatization. For bud break induction, the number of sprouted buds and the percentage of tubers with sprouted buds were evaluated. For in vitro establishment, different concentrations of MS culture medium were compared with respect to the percentage of establishment and contamination. In the multiplication stage, the influence of KIN and 6- BAP on the number of leaves, height, number of shoots and number of axillary buds was analyzed. In the rooting stage, the influence of ANA and AIA on the number and average length of aerial and submerged roots was evaluated. For the ex-vitro acclimatization stage, mixtures of peat, perlite and sand were analyzed with respect to variables such as survival percentage, number of internodes, number of leaves and degree of root ball emergence. In the induction phase, there were no differences between AG3 concentrations and immersion time as in the establishment phase, although the lowest percentage of contamination was obtained with 100% DM and 50% DM; the highest percentage of establishment was with 100% DM. In the in vitro multiplication phase, concentrations of 2 mg/L and 4 mg/L kinetin increased the values of all variables at 7 days. With respect to 6-BAP concentrations, concentrations with 2 mg/L and 3 mg/L 6-BAP positively influenced all variables except number of shoots. In the rooting stage, there were no differences between AIA concentrations and for ANA there was only influence of the concentrations 0.01 mg/L and 0.02 mg/L for the variable number of aerial roots. Finally, in the ex-vitro acclimatization stage, there were no differences between the different peat + perlite + sand mixtures. With the results of the present investigation, a method for micropropagation of plants of S. tuberosum L. Var. Superchola using buds sprouted from tubers by direct organogenesis, in the Plant Biotechnology laboratory of the Faculty of Agricultural Sciences of the Technical University of Ambato.