Variabilidad de la región its2 en poblaciones de Tetranychus urticae Koch en el cultivo de fresa (Fragaria vesca)

Abstract

The ITS2 is one of the most important markers in molecular systematics and evolution, in addition it shows a significant sequence variability at species level or below. The objective of the present investigation was to determine the variability of the ITS2 region in DNA fragments of individuals from populations of T. urticae in three varieties of strawberry cultivation (F. vesca), thus carrying out the study in the Biotechnology laboratory of the Faculty of Agricultural Sciences. Female mites were used for DNA extraction, DNA amplification by the ITS2 protocol for PCR that was performed in a thermal cycler. Obtaining the lengths of the ITS2 fragment for different populations of T. urticae, we counted 197 nitrogenous bases (A, T, C and G), of which are rich in A + T, with percentages of 68.53% in Festival (F ), 67% in Monterrey (M) and 68.02% in Albion (A). In total, 12 substitutions were found in the sequences of base pairs with 6.09%; therefore, in the differences of the sequences, 11 substitutions were found between Festival and Monterrey (F - M) with 5.58% divergence and 94.42% similarity, between Festival and Albion (F - A), 4, 57% divergence and 95.43% similarity, and between Monterrey and Albion (M - A) 2.03% divergence and 97.97% similarity. Proving that the percentages of Nitrogenous Bases in Monterrey (M) is less than F and A, due to environmental factors external to the molecular structure.