Estudio comparativo de los métodos: Electro Quimioluminiscencia, e Inmunofluorescencia. Para la determinación de Troponina Cardiaca como ayuda diagnóstica en el Infarto Agudo de Miocardio

Abstract

The comparative study of analytical methods is a new and innovative research, this research was conducted in the laboratory Movilab SA, of Ambato and in the laboratory of the Regional Hospital of Ambato, whose objective was the determination of cardiac troponin using two methods different as: the method electrochemiluminescence used by the cobas 411 equipment and that determines troponin Cardiac TnT and immunofluorescence method used by the I-Chroma team determines Troponin Cardiac TnI and identify which method is more sensitive and specific to help diagnose IAM.

The research was analytic observational with case control study because its goal was to test the hypothesis and to discover which of the two methods is the most sensitive to aid diagnosis of Acute Myocardial Infarction.

For sampling, we verified that the participants meet the criteria for inclusion, as the type of sampling is probabilistic and the choice of patients was on a scientific basis. The data obtained by the determination of the two methods gave quantitative results, the study population included 52 patients aged between 40-80 years of service Regional Emergency Hospital of Ambato, with medical application of cardiac troponin determination.

It was concluded that the sensitivity for electrochemiluminescence method was 41% with a specificity of 100% for patients diagnosed with IAM, while the immunofluorescence method sensitivity was 23.8% with a specificity of 84% for patients diagnosed with IAM.