Identificación genotípica y análisis de prevalencia de Mycobacterium kansassii en muestras de pacientes con sospecha de tuberculosis pulmonar que asisten al centro de Salud # 1 de la ciudad de Ambato – Tungurahua

Abstract

My present research work was carried out at the Health Center No. 1, in the city of Ambato, Province of Tungurahua, with samples from patients with suspected pulmonary tuberculosis, my study is aimed at investigating the genotypes and prevalence analysis of Mycobacterium kansasii. Taking into account that in recent years, M. kansasii has emerged as one of the main atypical mycobacteria causing disease in man, with its most frequent clinical manifestation being lung disease. Generally, it is sensitive to classic tuberculostatics, and therapeutic failures are usually associated with resistance to rifampicin. Although phenotypically it is a very homogeneous species; there are different studies that describe different biotypes. In addition, in recent years the existence of seven genotypes (I to VII) has been described, of which genotype I is the most prevalent and the main cause of disease in man. For all these reasons, in this work I set out to know the genotypes of M. kansasii circulating in the area of influence of the Health Center No. 1, for which we carried out a genotypic characterization that showed a high homogeneity among our patients, being mostly included in a biotype; the detection of M. kansasii-specific DNA probes that confirmed species identification and reverse hybridization techniques that determined that all our isolates belonged to genotype I.