“Actividad anti-metastásica y anti-proliferativa de los extractos de plantas ilex guayusa, uncaria tomentosa y croton lechleri, en la línea celular mcf7 de cáncer de mama”

Abstract

Breast cancer is the second leading cause of death in women worldwide, among the most common neoplasms in the female sex followed by cervical, thyroid, stomach and colorectal cancer; breast cancer is the neoplasm that has the greatest impact in Ecuadorian women. In this context, Ecuador is a country with a great biodiversity in flora and ethnicities, where plants have been used over time as a treatment for various diseases. Ancestral knowledge and the use of plants in the country are transmitted empirically, hence the importance of derived research that provides a scientific sustenance of their effects. The objective of the research was to evaluate the anti-proliferative and anti-metastatic effect of Ilex guayusa, Uncaria tomentosa and Croton lechleri extracts on the MCF-7 breast cancer cell line. The extracts were obtained by maceration in 96% ethanol, distillation, freeze-dried and resuspended in PBS 1X. I know used the MFC-7 cell line for the surface adhesion-dependent clonogenic assay, as well as for the assay called Soft Agar, each assay consisted of evaluating the effect of various concentrations of the extracts, with such concentrations being 3 µg/µL, 0.3 µg/µL and 0.03 µg/µL. The results showed that several of the extracts analyzed possessed an anti-proliferative activity on MCF-7, with Croton lechleri extract having the greatest effect in inhibiting cell proliferation. Furthermore, the Soft Agar trial, made it possible to determine that the extracts of Ilex guayusa had an anti-metastasic activity dependent on concentration, the higher concentration effect; Uncaria tomentosa and Croton lechleri had a greater effect in inducing metastasis, where total effectiveness was observed in dilutions 3 µg/µL and 0.3 µg/µL; presenting in the dilution 0.03 µg/µL of Croton lechleri a significantly positive anti-metastasic activity compared to Positive Control. The results were analyzed by the analysis of variance (ANOVA) Conpos Test de Tukey, obtaining a significance of ≤ 0.05 which allows us to accept the alternating hypothesis.