Ciencia e Ingeniería en Alimentos y Biotecnología
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Item Validación del método analítico por espectrofotometría uv-vis para la determinación de edulcorantes en bebidas azucaradas consumidos en la provincia de Tungurahua(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03) Castillo Mayorga, Alison Abigail; Pérez Aldas, Lander VinicioThe present research focuses on the validation of the calibration curves of the UV Vis spectrophotometry analysis method for the detection of synthetic sweeteners of sucralose, aspartame and acesulfame K, the study is aimed at possible applications in sugary drinks. For the analysis, standard solutions were prepared with buffer and chromophore solutions in known concentrations. The study was carried out in a UV Vis Thermo Scientific Evolution 60S series spectrophotometer. The calibration curves were made with the proposed concentrations for each standard solution with which the value of R squared was determined, in all cases it was greater than 99 percent, in turn, the equations of calibration for each solution. A statistically significant linearity was determined for the process and a sensitivity of 0.0252; 0.0018 and 0.2332 for sucralose, aspartame and acesulfame K. The detection limit calculated for each standard solution corresponds to 0.0547, 0.0249 microgram per milliliter and 0.0459 milligram per milliliter for sucralose, aspartame, and acesulfame K, respectively; the calculated expanded uncertainty was between 7.44, 19.08 and 21.5 percent for sucralose, aspartame and acesulfame K, respectively. In turn, through a bibliographic review, it was possible to determine that HPLC chromatography is a method for detecting high precision synthetic sweeteners with very sensitive detection limits, however, spectrophotometry is also a good option for this type of analysis that presents a great sensitivity.Item Validación del método para determinación de detergentes en muestras de agua por espectrofotometría UV-Vis en el laboratorio AqLab de la ciudad del Coca(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2022-03) Aguirre Tubón, Ana Belén; Pérez Aldas, Lander VinicioThe validation of the method for determination of surfactants in water samples by UV-vis spectrophotometry was carried out in the environmental analysis and evaluation laboratory Aqlab in the city of Coca. Its validation was carried out taking into account the analytical need of the laboratory, validation parameters of the INEN ISO IEC 17025 standard and SAE regulations, environmental regulations described in the TULSMA, in its Environmental Quality and Effluent Discharge Standard: Water Resource and the Municipal Ordinances of the Orellana Canton. The method 5540 C corresponding to the determination of anionic surfactants as MBAS described in the Standard Methods was used for the matrices of water for human consumption, natural water and waste water. The validation objectives were established and the corresponding experimental design was proposed under conditions of repeatability and reproducibility. The following parameters were analyzed: Response Function, Limit of detection, Limit of quantification, Precision (repeatability and reproducibility), Veracity, Uncertainty and Working Interval of the method. Through the statistical analysis of data, it was demonstrated that the method is suitable for the intended application, by meeting the validation objectives set for a CVr and CVR less than 30 percent for accuracy, a recovery percentage of 80 to 120 percent for veracity, an uncertainty U less than 30 percent with a 95 percent confidence interval (k is 2) and a method working range comprising concentration levels 0,10 to 8,85 ppm MBAS.