Ciencia e Ingeniería en Alimentos y Biotecnología

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Subject: search.filters.subject.CÁNCER DE MAMA

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    Evaluación in silico de la influencia de la heterogeneidad tumoral en la evolución clonal del cáncer de mama
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Caicedo Albán, Samantha Lizbeth; Galarza Galarza, Cristian Fernando
    The present study seeks to understand the influence of tumor heterogeneity on cancer progression and metastasis development, proposing a starting point for personalized treatment. Data from breast cancer were used, with sequences from samples belonging to primary tumors and tumors in the lymph nodes. Bioinformatics techniques were applied for processing, and differential expression analysis was used to identify genes that were expressed in each of the experimental conditions. Interaction networks were represented to identify the different interactions of elite genes with other genes. The functionality of these genes was validated using the functional enrichment technique to identify the role that these genes play in cell integrity and homeostasis. A search for variants was performed with the selected genes to identify the influence that these genes have on the development and progression of metastasis. Tumor heterogeneity on clonal evolution was evaluated by analyzing the influence of the variants of each type of tumor, observing that GL has a higher incidence on metastasis compared to TP.
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    Capacidad proapoptótica in vitro de Valeriana rígida y Valeriana decussata en células cancerígenas
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01) Williams Ibarra, Jeniffer Andrea; Carrero Castillo, Yenddy Nayghit
    Cancer represents a public health problem level world, with high incidence and mortality rates in developed and undeveloped countries. Currently there are different therapeutic treatments to eliminate or prevent the proliferation of cancer cells, however, the most are invasive, and they have side effects. Today there are natural therapeutic alternatives with the aim of develop more efficient and less invasive treatments to fight cancer. Faced with this problem, the research done it is based on in vitro evaluation of the proapoptotic and cytotoxic capacity of valerian extracts on the breast cancer cell line. Cell viability assays (MTT), immunohistochemistry assays were performed by fluorescence microscopy and quantitative reverse transcription PCR for gene expression of proteins. The results obtained showed the decrease in cell proliferation and variability in the gene expression of apoptotic proteins in breast cancer cells after being treated with optimal doses of Valeriana rigid and Valeriana decussata extracts.
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    Posible papel estrogénico de metales pesados en cultivos de células de cáncer de mama MCF-7
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-07) Cabrera Riofrío, David Alejandro; De Lucca Osorio, Marisel
    Estrogens could act as an important risk factor in the development of breast cancer, an increase in estrogen produced in our body, or an overexposure to environmental estrogens such as heavy metals cause a signal cascade that leads to the overactivation of oncogenic genes (cfos, c-myc) through estrogen receptors (ERs). This mechanism promotes the development of malignant phenotype, by altering the cell cycle leading to the formation of tumors. The main objective of the present project was to evaluate the estrogenic role of heavy metals in cultures of MCF-7 breast cancer cells to establish an experimental model that allows us to study the alterations that occur at the cellular level when exposing these cells to different metal concentrations. The effect of different concentrations of heavy metals (chromium, lead, mercury, nickel, cadmium and arsenic) on MCF-7 cells was analyzed by its action on cell proliferation and estrogen receptor gene expression alpha (RE-α). In addition, the binding of these metals to this receptor was examined by competition studies in the presence of estradiol-FITC, through fluorescence microscopy techniques. The results indicate that these heavy metals have an estrogenic effect on MCF-7 cells, and this effect exerts it through their binding to RE-α