Ciencia e Ingeniería en Alimentos y Biotecnología

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Subject: search.filters.subject.BOTRYTIS CINEREA

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    Evaluación de la actividad antifúngica de un microencapsulado rico en polifenoles del residuo de caña de azúcar (Saccharum officinarum L) frente a Botrytis cinerea
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Abril Díaz, Viviana Gabriela; López Hernández, Orestes Darío
    Currently, biotechnology is looking for alternatives to replace commercial fungicides in the control of pathogens such as Botrytis cinerea, responsible for approximately 10 percent of annual crop losses. Research on the use of plant residues, whose metabolites fulfill this purpose without causing harm to the environment or humans, have emerged as central areas of study. Such is the case of sugarcane bagasse, rich in polyphenols with antifungal activity, being capable of being used in the future within the fields as a pest control agent. To obtain the extract rich in polyphenols, the organic solvent extraction method was used using ethanol at 30, 50 and 70 percent evaluated at 30, 45 and 60 minutes in a 1:20 plant material:solvent ratio. After this, the Folin test was performed to measure the concentration of total polyphenols, the best treatment being 50 percent at 30 minutes for 63 g per GAE. Next, microencapsulation of the concentrate was carried out at a 20:80 ratio using maltodextrin, using the spray drying technique, and its efficiency was evaluated using FTIR. Finally, the in vitro antifungal activity was evaluated at 10, 30 and 50percent microencapsulation as an antibiogram, obtaining a higher percentage of inhibition at 50 percent microencapsulation than the total average of the 8 days of the trial.
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    Evaluación de la capacidad antagónica de Trichoderma harzianum microencapsulada frente a Botrytis cinerea
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Guevara Freire, Jennifer Michelle; Pérez Salinas, Ruth Narcisa
    The blackberry crop has a high economic importance due to its medicinal and agroindustrial properties, its production faces problems caused by phytopathogens that cause significant economic losses, among which Botrytis cinerea stands out as the cause of gray rot disease. The control of this fungus has been demonstrated by organisms such as Trichoderma harzianum, however, the elaboration of products based on this biocontroller is unstable or inefficient, being microencapsulation an excellent alternative. For research purposes, conidia of T. harzianum were obtained by solid fermentation and these were microencapsulated by spray drying technique from maltodextrin and gum arabic, ionic gelation was performed by using sodium alginate. Two types of microencapsulation were used, as well as three incubation times, obtaining as a result that the ionic gelation treatment at three days of incubation was the best with a percentage of radial growth inhibition of 97.087 percent followed by ionic gelation at 2 days with a percentage of 94.174 percent, while the spray-drying treatment at 3 days was 85.435 percent. In the present research project, microencapsulation of T. harzianum conidia was carried out, evaluating its influence on the radial growth percentage of B. cinerea.