Ciencia e Ingeniería en Alimentos y Biotecnología

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Subject: search.filters.subject.ANÁLISIS MICROBIOLÓGICO

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    Verificación de la aptitud del método microbiológico para tres matrices farmacéuticas (sólida, semisólida y líquida) del laboratorio Neofarmaco del Ecuador Cía. Ltda.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2022-03) Rivadeneira Cueva, Brenda Patricia; Calero Cáceres, William Ricardo
    This degree project describes the verification of the suitability of the microbiological method for non-sterile products, which includes tests for the presence and absence of the most common specific microorganisms such as: Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, Candida albicans; and total aerobic (Bacillus subtilis) and mold-yeast (Aspergillus niger) count tests with certified ATCC strains. The results of the microbiological analyzes were evaluated according to the criteria established by USP and AEFI for each of the three pharmaceutical matrices: solid (chewable tablets), semi-solid (vaginal cream) and liquid (pediatric drops). For the verification of the method, it was evaluated under certain parameters, where it is highlighted that it is exact since it yielded percentages greater than 70 and that it was found within the range established by the USP, not less than 50 nor greater than 200 percent of microbial recovery; accurate because the data was less than 20 percent and robust. In addition, in the robustness test, factors such as temperature, incubation time and type of plate that can influence the growth of the counting microorganisms were compared. According to the results, it is shown that the verified microbiological method is reliable and appropriate for the determination of pathogenic microorganisms and the count of total aerobes and molds-yeasts in the three pharmaceutical matrices of the Neofarmaco Laboratory of Ecuador Cía. Ltd.
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    Determinación de la correlación entre métodos visuales ópticos y difusión en placa en el crecimiento de Escherischia coli
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-09) Fiallos Núñez, Johanna Elizabeth; Dugarte Jiménez, Nahir Yerely
    The correlation between optical methods: Spectrophotometer and turbidimeter, visual method: McFarland scale and the reference method of plate diffusion were evaluated in order to determinate the concentration of Escherichia coli at 9 hours. The visual method doesn´t have a correlation with the reference method because is a qualitative method and the measurements are subjective. In the case of the optical methods, the spectrophotometer had an error of 91,4 percent in comparison of the turbidimeter with an error of 2,2 percent. The turbidimeter was the only method which has a correlation with the reference method and can be used as an alternative for the traditional plate diffusion method.
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    Desarrollo de un método para recuento de Listeria monocytogenes utilizando turbidimetría
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-09) Bustos Cosios, Cristina Verónica; Arancibia Soria, Mirari Yosune
    The present study was performed to develop a method for recounting Listeria monocytogenes using turbidimetry, the proposed method consists in a scale of four equidistant points, prepared with sulfate of sodium and chloride of barium that produce a precipitate of sulfate of barium, which resembles cellular concentration of 10 x 108 UFC/ml to 40 x108 UFC/ml, innovating the scale of turbidity proposed by McFarland in 1907. Using the proposed scale of precipitate of sulfate of barium, an interpolation equation was developed in which readings of the nephelometric turbidity units of microorganisms in culture medium are applied, predicting in a mathematical way the cell concentration in a liquid culture medium. The error rate of this method compared to the plate count method is 0,28 to 3,41 percent. Of the above is possible to use the turbidimeter to predict cell concentration effectively, simplifying microbiological management in investigative procedures, without the need for long preparation time to know the cellular concentration.