Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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2019 - 201912020 - 20212

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Tema: search.filters.subject.PIGMENTOS LIPOSOLUBLES

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  • Miniatura
    Item type:Ítem,
    Optimización del proceso de extracción de carotenoides obtenidos a partir de la flor de mastuerzo (Tropaeolum majus L)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01)
    Amancha Laura, Alex Giovanni
    ;
    López Hernández, Orestes Darío
    The present investigation was based on the optimization of the operational parameters that influence the extraction of carotenoids from the orange flower of the mastuerzo (Tropaeolum majus L) through the response surface methodology (MSR). Considering that these metabolites are fat-soluble organic pigments, a solid-liquid extraction with 96 percent ethanol was carried out, by studying two factors: the ratio of plant matter: volume of solvent (1:20, 1:45, 1: 70) and the extraction time (30, 60, 90 minutes). Subsequently, the carotenoid content was quantified by UV-Visible spectrophotometry, determining that the best treatment corresponds to 70 parts by volume of ethanol in a time of 90 minutes, registering a value of 929.44 mg per 100 g of dry base, while the lowest value of 487.05 mg per 100 g was obtained with 20 parts of solvent in a time of 30 minutes. Subsequently, the MSR was applied, through which the levels of each of the factors were optimized, determining that the optimum point corresponds to 70 parts of ethanol volume in a time of 90 minutes, reaching a maximum concentration of 940.92 mg of carotenoids per 100 g, with an extraction efficiency of 0.18 percent and a desirability of 0.97. Finally, a vacuum distillation was developed, obtaining the concentrated extract of carotenoids with a humidity of 80.59 percent, which was stored at 4 degrees Celsius for its conservation, since it has antioxidant potential.
  • Miniatura
    Item type:Ítem,
    Extracción y microencapsulado de carotenoides con actividad antioxidante a partir de Pouteria sapota
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2020-01)
    Guevara Gonzalez, Jarol Damian
    ;
    López Hernández, Orestes Darío
    It is well known that carotenoids are compounds that have a high antioxidant power and their contribution to the human diet has great health benefits. The present research work focused on the extraction, microencapsulation and analysis of carotenoids present in mamey zapote (Pouteria sapota) for its antioxidant properties, based on an experimental surface design, so it was determined that the optimal conditions were 30 minutes with a vegetable-solvent mass ratio of 1:67 using 96 percent ethanol as solvent at a temperature of 70 Celsius degrees. To preserve the carotenoids against external factors, the microencapsulation process was carried out by the spray drying method, with a polymeric mixture of maltodextrin and gum arabic in equal proportions. The efficiency of the microencapsulation process was analyzed by FT-IR infrared spectrophotometry, the spectra obtained demonstrated the protection of the polymer mixture to antioxidants. The antioxidant activity was determined by an in vitro assay by the DPPH method, obtaining equivalent Trolox values of 253,21 micromoll-1 for the concentrated extract and 385,19 µmoll-1 for the microencapsulation. In addition, the in vivo antioxidant activity was analyzed through the Saccharomyces cerevisiae growth curve of the microencapsulate at a concentration of 50 mg for ml with maximum absorbances of 1,259 and 1,335 for 3 mmoll-1 and 0,5 mmoll-1 hydrogen peroxide respectively compared to vitamin C as an antioxidant model.
  • Miniatura
    Item type:Ítem,
    Extracción y microencapsulación de carotenoides con actividad antioxidante a partir de Daucus carota
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2019-07)
    Fiallos Yánez, Henry Alexis
    ;
    López Hernández, Orestes Darío
    The present research work was based on the extraction and analysis of metabolites of the Daucus carota plant for its antioxidant properties, taking as a starting point the extraction of carotenoids based on an experimental surface design. To take advantage of the properties of the extracted metabolites, microencapsulated microcapsules containing the antioxidants of the extract of the Daucus carota plant were developed, using two saccharide-type polymers which were maltodextrin and gum arabic of great commercial use with which a microencapsulation efficiency of 92.21 percent was obtained, which were used to verify the antioxidant properties by means of in vitro tests by the DPPH method to analyze the percentage of inhibition of free radicals in which 394,076 μmol equivalents of Trolox was obtained. A percentage of inhibition of 71.20 percent for the microencapsulation, and in the antioxidant activity in vivo through the growth curve of the yeast Saccharomyces cerevisiae the antioxidant potential was determined.