Ciencia e Ingeniería en Alimentos y Biotecnología
URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412
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Item type:Ítem, Caracterización biológica de andamios elaborados con nanocelulosa vegetal y quitosano. Articulado al proyecto de investigación aprobado mediante la Resolución Nro. UTA-CONIN-2025-0029-R(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-07) ;Páez Vayas Sebastián PatricioNúñez Villacís Lorena de los ÁngelesThis research focuses on the study of biological scaffolds, which are considered three-dimensional structures that mimic the support of the extracellular matrix, facilitating cell proliferation and adhesion. The main objective was the biological characterization of scaffolds made from plant-derived nanocellulose and chitosan through hemostatic assays. The methodology was experimental, since the hemostatic indices of the proposed scaffolds were previously unknown. Therefore, tests were conducted for the first time, varying the concentration of the polymer solution to determine the optimal formulation for scaffold development as a three-dimensional model for culturing prostate cancer cell lines. The study followed a mixed-methods approach: qualitatively, the scaffolds were evaluated in terms of shape, consistency, and size; quantitatively, six hemostatic tests were conducted—degradation, maximum swelling, coagulation index, hemolysis, red blood cell adhesion, and platelet adhesion. Results showed that scaffolds with higher chitosan concentrations exhibited better structural integrity and more efficient performance in the assays, whereas those with lower concentrations were fragile and displayed suboptimal ranges for use as 3D models in cell culture.Item type:Ítem, Evaluación de la capacidad de biodegradación de contenedores fabricados a partir de recursos agroindustriales infrautilizados de la industria bananera(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) ;Méndez López, Tomás SebastiánArancibia Soria, Mirari YosuneAccording to the United Nations, approximately 430 million tons of plastic are produced each year. Consequently, this waste represents 85 percent of the total contamination in bodies of water, 36 percent of which are disposable containers. To combat this problem, resources have been focused on the research of more environmentally friendly materials. In Ecuador, banana, due to its high production and waste generation, emerges as one of the best alternatives for the development of bio containers to replace single-use plastics. For this reason, this research evaluated the biodegradation capacity of containers made from underutilized agro-industrial resources of the banana industry. For this purpose, a buried test was carried out with samples of biodegradable banana containers (CBB) in agricultural soil for 15, 30, 45 and 60 days, where values of up to 52.53 percent biodegradation based on dry weight were obtained. On the other hand, in vitro biodegradation capacity was evaluated by inoculation of Aspergillus niger, where it was demonstrated that this strain prefers CBB as a substrate, compared to filter paper and single-use plastics, due to the greater availability of nutrients for its growth. Finally, through microbiological analysis and bibliographic research, it was demonstrated that the shelf life of CBB is equal to or greater than 60 days, since their physicochemical and usability characteristics are not compromised during this period.Item type:Ítem, Extracción de nanocelulosa vegetal a partir de residuos de banano (Musa paradisiaca) y su aplicación en andamios biológicos(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) ;Núñez Martínez, Kimberlyn CarolinaNúñez Villacís, Lorena de los ÁngelesThe wound healing process is highly complex and alterations in its pathway can cause severe scenarios with high economic cost. Tissue Engineering (TE) combines cells, scaffolds, and growth factors to give rise to new tissue. Scaffolds function as a support for cell growth and are fabricated with synthetic or natural biopolymers. Nanocellulose is a natural polymer that can be obtained from renewable sources, such as banana waste. This study focused on the extraction of nanocellulose from banana waste and its subsequent application in the fabrication of biological scaffolds for future research in the healing of chronic wounds. Banana nanocellulose was extracted by hydrolysis with sulfuric acid and ultrasound. Its morphology was evaluated by inverted light microscopy and SEM. In addition, the main functional groups present in nanocellulose were analyzed by FTIR. Subsequently, nanocellulose, chitosan, and nanocellulose whit chitosan scaffolds were prepared by freeze-drying in order to evaluate the characteristics of nanocellulose versus other biopolymers. Scaffolds were then evaluated by SEM, porosity tests, water absorption capacity and biodegradability tests. A yield of 23.8 percent of extracted nanocellulose was obtained. The scaffolds composite of nanocellulose and chitosan showed the best results with an average pore size of 110.13 micrometers with 88.94 percent. porosity, 2418.32 percent of water adsorption and an ability to degrade in 16 days. The results, suggest that these scaffold have potential to be applied in TE research related to wound healing and tissue regeneration.Item type:Ítem, Estudio de las propiedades fisicoquímicas y funcionales de la harina de banano (Musa acuminata AAA) de rechazo en el desarrollo de películas biodegradables.(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería en Alimentos., 2016-04) ;Ortega Alvardo, Johana ElizabethEcheverría, IgnacioThis work is about physicochemical and functional characterization of flour of banana (Musa acuminata AAA) for rejection and Cavendish cultivate, it made with banana from two provinces (Los Rios & El Oro) the highest producers of Ecuador. On this study was evaluated the effect of production zone, husk incorporation and use of sodium metabisulfite, getting 8 treatments with a yield between 21.16 por ciento and 29.85 por ciento.Inside the physicochemical properties of banana flour evaluated, the whiteness index was influenced by the use of metabisulfite, obtaining values between 56.91 to 76.49; the highest values were the flour with metabisulfite. Also was evaluated total content of starch, amylose and crude fiber in flours, resulting ranges from 67.48 to 78.21 por ciento, 12.82 to 17.98 por ciento and 1.15 to 3.50 por ciento respectively. These contents influenced mainly in the functional properties of banana flour at 80 Celsius degree. The optical properties of the biodegradable films show variation in parameters such as the whiteness index (57.05 to 77.72), yellowness index (11.66 to 81.06) and color difference (9.75 to 49.17); obtaining films with a higher whiteness index in treatments using sodium metabisulfite and a differential color and yellowness index highest for treatments without metabisulfite due to enzymatic browning. The mechanical properties, such as puncture force and puncture distance of biodegradable films showed a range of values between 15.32 to 25.97 N and 10.42 to 13.60 mm respectively, greater resistance was observed in treatments with high fiber. Samples with higher puncture distance were those that had a low fiber content and high starch content. As puncture force, barrier properties as permeability to water vapor (3,16 x10-10 – 6,89 x10-10 g•m-1•s-1•Pa-1) and solubility (50.53 to 60.02 percent).Item type:Ítem, Utilización de harina de plátano (musa balbisiana), en el desarrollo de películas biodegradables activas(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería en Alimentos, 2015) ;Moreno Toasa, Gabriel AlejandroArancibia Soria, Mirari YosuneItem type:Ítem, Elaboración de galletas con una mezcla de harina de banano (musa cavendishii), harina de trigo y glucosa(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería en Alimentos, 2013) ;Gallegos Chango, Alexandra MargaritaGerman Tomalá, César Augusto
