Ciencias Agropecuarias

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    Efecto del agua ozonizada sobre el establecimiento in vitro de segmentos nodales de S. betaceum Cav
    (2025-02) Tibanquiza Bastidas Wilmer Ismael; Leiva Mora Michel
    The study of the effect of ozone water on the in vitro establishment of nodal segments of S. betaceum. Cav, focuses on the evaluation of sustainable and effective ways to improve the propagation process in plant tissue culture. S. betaceum. Cav, also known as tree tomato or tamarillo. Tomato is a useful and commercially valuable crop that can be propagated in vitro to obtain genetically modified plants free of diseases and pests. Ozone water, with its antimicrobial and oxidative properties, has proven to be a good tool to disinfect plants by reducing the risk of pathogenic microorganisms without affecting cell survival. This method can replace traditional disinfectants such as sodium hypochlorite, chlorine dioxide, hydrogen peroxide, etc., which have many phytotoxic effects. However, their use on S. betaceum. Cav has not been thoroughly investigated. The use of ozonated water in the composition of parts of S. betaceum. Cav in vitro has a positive effect on plant growth and development. Ozone, because of its antimicrobial properties, helps to control microbial contamination in culture media, thus improving plant health and well-being. In addition, it increases nutrient uptake, stimulates root growth and regenerates the shoot. However, the correct concentration of ozone must be determined to avoid toxic effects, thus achieving a balance between benefits and plant protection. The main objective of this method is to optimize pure conditions and facilitate the successful formation of hybrids thus providing a basis for successful hybridization of this type. The objective of this research is to promote innovation in plant biotechnology and the development of sustainable practices.
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    Evaluación del efecto del agua ozonizada sobre el porcentaje de contaminación y de establecimiento in vitro de segmentos nodales de Physalis peruviana L. Var. manzana
    (2025-02) Castro Nuñez Richard Johao; Leiva Mora Michel
    The research work had the objective to evaluate the effect of ozonized water as a disinfectant agent on the percentage of contamination and in vitro establishment in Physalis peruviana L. during the establishment phase. The trial analyzed different concentrations of ozonized water for the disinfection of explants of Physalis peruviana L. used concentrations of 1.2 and 3 ppm of ozonated water were used in an immersion time of 5 minutes. Results were obtained in which no concentration managed to eliminate microbial agents, giving a contamination percentage of 100% and an establishment of 0%. In the test in the results there was only fungal contamination such as Penicilium sp., Cladosporium sp., Fusarium sp. y Colletotrichum sp., no bacterial contamination was observed, suggesting that ozonated water may have a disinfectant effect on bacteria.