Ciencias Agropecuarias
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Item Determinación de la titulación de anticuerpos post vacunales en Leucemia Viral Felina mediante ensayo inmunoenzimático de tipo indirecto en gatos de diferentes edades(2024-02) Viteri Ortiz, Rosa Noemi; Kelly Alvear, Gerardo EnriqueFeline Viral Leukemia (ViLeF) is a retrovirus that affects domestic cats to a great extent, causing persistent infections and even death; however, vaccination as a means of prevention avoids the contagion and spread of the virus. For this reason, the research focused on the identification of post-vaccination antibodies in domestic felines by means of an indirect enzyme-linked immunosorbent assay using plasma extracted before and after the application of the vaccine, to identify the increase or decrease of post-vaccination antibodies. According to the results obtained, no statistically significant differences were found in the amount of preand post-vaccine ViLeF antibodies where the scores of the pre-vaccine results of the amount of antibodies (M= 0.06; SD= 0.03) were higher than the scores of the post-vaccine results of the amount of antibodies (M= 0.05; SD= 0.01) t(24)= 1.52, p˂0.005, d= 0.14, d being greater than p value. We sought to identify the relationship between cases positive for ViLeF with factors such as sex, age and reproductive status, omitting the animal's lifestyle, since the samples were obtained from 25 cats rescued by the Municipal Shelter of the city of Ambato. However, the mentioned variables did not show any relation with the increase of antibody titer against ViLeF in domestic cats, where the results obtained in relation to sex showed that the difference of means of both pre-vaccination (M=0.08) and post-vaccination (M=0.05) results in males was 0.03, being the same a negative variation and in females, the means of pre-vaccination (M=0.05) and post-vaccination (M=0.05) results did not show any difference of means. According to age, the results are: 6 - 11 months before - vaccination (M=0.06) and post - vaccination (M=0.05); 1 - 3 years before - vaccination (M=0.06) and post - vaccination (M=0.05) and 4 - 8 years before - vaccination (M=0.06) and post - vaccination (M=0.05), the difference was negative M(dif)= 0.01 in all ranges. Finally, the influence of reproductive status was evaluated and the following results were obtained: Sterilized before-vaccination (M=0.06) and after-vaccination (M=0.05) and Whole before-vaccination (M=0.06) and after-vaccination (M=0.05), in both cases, the variation was -0.01. Key words: Feline viral leukemia, retrovirus, domestic cats, vaccine, post-vaccination antibodies, immunity, ELISA