Ciencias Agropecuarias

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    Efecto in vitro de Beauveria bassiana en el control de Melophagus ovinus (Diptera: Hippoboscidae)
    (2024-08) Córdova Córdova, Johanna Lissette; López Villacis, Isabel Cristina
    Melophagus ovinus is an ectoparasite of sheep able to cause weight loss, anemia, and reduced wool growth, leading to an economic impact due to reduced production of meat, milk, and wool from sheep, as well as damage caused by the transmission of zoonotic pathogens, thus requiring permanent control measures. In this research, in vitro effect of Beauveria bassiana on the control of Melophagus ovinus was evaluated. For this purpose, the effect of four doses of the biopesticide (T1 = 1.0x105 spores/ml; T2 = 1.0x106 spores/ml; T3 = 1.0x107 spores/ml; T4 = 1.0x108 spores/ml, and distilled water was used as control) was tested on the mortality rate of adult female M. ovinus. Additionally, LD50 was calculated using Probit analysis. Concentrations of B. bassiana affected the mortality rate in M. ovinus. In general, the mortality rate was dependent on the concentration of B. bassiana, reaching 55.56% at 120 hours after applying the highest dose, which was 50% higher than the mortality observed with the lowest dose and 90% higher than the control treatment. A similar trend was observed in the second and third evaluations. Probit analysis showed an LD50 value of 1.47x105 conidia/ml, while the LD90 and LD95 values were 6.7x106 and 3.8x107 conidia/ml, respectively, which it would be expected to result in 90% and 95% mortality of the study population under in vitro conditions. According to the results, topic application of B. bassiana suspensions could be a potential alternative for controlling M. ovinus; however, in vivo evaluations are required to determine its effect on sheep.