Ciencias de la Salud
Permanent URI for this communityhttp://repositorio.uta.edu.ec/handle/123456789/813
Browse
1 results
Search Results
Item Expresión de las proteínas bax y bcl2 en lesiones preinvasivas e invasivas de cérvix.(Universidad Técnica de Ambato/Facultad de Ciencias de la Salud/Carrera de Medicina, 2021-02-01) Mestanza Zurita, Jéssica Gabriela; Carrero Castillo, Yenddy Nayghit Dra. PHDIntroduction: Cervical cancer represents 7.5% of female mortality worldwide. Late detection, lack of knowledge about risk factors, about detection tests and their difficult access are the main causes. Neoplastic processes in cervix are triggered by the Human Papilloma Virus (HPV) 16 and 18, as well as the progression to malignancy whereby the application of costeffective prevention models such as screening and opportune treatment of preinvasive lesions is essential. The mechanisms and factors which determine the progression from preinvasive to invasive lesions remain a matter of debate. However, strategic HPV action points have been identified in the mechanisms of infection persistence, evasion and alteration of the immune system; and the modification of apoptotic pathways, all of these for the purpose of cell immortalization carried out through the alteration of protein 53 (p53) and Retinoblastoma protein (pRb) and, therefore, in protein signaling of the BCL2 family, essential proteins in the regulation of cellular apoptosis for tissue homeostasis, as well as in the cellular mechanisms of protection against DNA damage, cellular stress or harmful stimuli, and one of the main targets of HPV so their interaction is the subject of several investigations. Objective: Analyze the expression of BAX and BCL2 proteins in pre-invasive and invasive cervical lesions. Methodology: The study population was composed of female patients who met the inclusion criteria, a clinical history, gynecological physical examination, taking Papanicolao samples, colposcopy and biopsies that were later processed by immunohistochemistry. In addition, HeLa cell cultures were performed, from which the RNA was extracted and qRTPCR was performed for BAX and BCL2. Results: BAX presented statistically significant in situ expression when comparing the control vs CIN I, II, III and cancer, but no relationship was found between the progression of the lesions. On the other hand, BCL2 did not show significant expression values. In the relative expression of Bax and Bcl2 by qRTPCR in Hela cells, BAX showed higher expression. No changes were identified in the expression of BAX and BCL2 proteins depending on the HPV positivity infection. Conclusions: BAX and BCL2 are pro-apoptotic proteins whose expression patterns are altered in situ and in peripheral blood lymphocytes due to their interaction with HPV, so these proteins are considered potential diagnostic and prognostic markers, hence the importance of their determination in samples from of patients with different degrees of injury. Recommendations: Carry out quantitative studies on the expression of BAX and BCL2 in pre-invasive and invasive lesions of the cervix and in peripheral blood lymphocytes to determine their predictive value in the progression of carcinogenic processes.