Maestría en Laboratorio Clínico Mención Microbiología Clínica
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Item ANÁLISIS DE LA SUSCEPTIBILIDAD A ANTIFÚNGICOS Y EVALUACIÓN DE FACTORES DE RIESGO ASOCIADOS EN CEPAS DE CANDIDA SPP. AISLADAS DE SECRECIONES VAGINALES EN MUJERES DE 22 A 65 AÑOS(Universidad Técnica de Ambato / Facultad de Ciencias de Salud / Centro de Posgrados, 2024-10-14) Alquinga Lovato, Jenny Carolina; Moina Veloz, Álvaro PaúlCandida spp. is a fungus that can be found in the vagina, causing vulvovaginal candidiasis (VC) causing gynecological problems in different ages, including those with diabetes, producing an impact on physical health, and their intimate relationships, commonly used as treatment azoles, the prognosis is good and curative, but if the predisposing factors of mycosis are not corrected a new reinfection occurs. Species identification was performed with CHROMagar candida and Biggy nickerson as control agar, in addition, antifungal susceptibility testing was performed along with a closed-question survey to identify risk factors associated with VC. To analyze antifungal susceptibility and evaluate the associated risk factors in Candida spp.strains isolated from vaginal secretions in women aged 22 to 65 years and to develop an algorithm for the identification and characterization of Candida spp. strains. The methodology has a descriptive, prospective, cross-sectional approach, involves the collection of a vaginal swab sample, the information and data were obtained during the course of the research. The results obtained represent the susceptibility profile circulating in this health center and could be potentially useful for monitoring and guiding treatment. When testing for susceptibility to antifungals C. albicans, C. parapsilosis were sensitive to fluconazole and voriconazole in 100%, C. glabrata was SDD for fluconazole, voriconazole has no cut-off point and C. krusei presented sensitivity to voriconazole, fluconazole shows intrinsic resistance. krusei presented sensitivity to voriconazole, fluconazole shows intrinsic resistance, within the risk factors we have the active sexual life, diabetes, which proposes to implement preventive measures to avoid recurrence of vaginal infections, for this reason it is necessary to expand the research in other populations such as pregnant women, patients with Human Immunodeficiency Virus, and minors.Item ESTUDIO DE LA ACTIVIDAD ENZIMÁTICA DE HEMOLISINA Y FOSFOLIPASAS DE CANDIDA SPP, EN AISLADOS CLÍNICOS DE PACIENTES QUE ACUDEN AL LABORATORIO COCAMED(Universidad Técnica de Ambato / Facultad de Ciencias de la Salud / Centro de posgrados, 2024-09-03) Preciado Marcillo, Evelyn Cristina; Echeverria Valencia, Gabriela FernandaMicroorganisms belonging to the Candida genus have evolved and over time have developed mechanisms of entry, persistence and pathogenicity to remain in the host, invade it and cause infection. There are several factors that favor pathogenicity by directly contributing to virulence, such as hemolysin and phospholipase activities. This work focused on determining the hemolysin and phospholipase activity of Candida spp, in clinical isolates from patients who come to the Cocamed clinical laboratory. The study is cross-sectional and descriptive, in which Candidas were identified by manual methods using chromogenic agar and automated methods such as Vitek, the hemolysin and phospholipase activity of Candidas was studied through specific plate media based on egg yolk and the other with lamb blood. In the results obtained from the Candida spp isolates, Candida albicans and non-albicans Candida were identified. Regarding phospholipase activity, C. albicans cultures had high and very high activity, and the remaining low/null activity, while non-albicans cultures had high and very high activity, and the remaining low/null activity. In Hemolysin, C. albicans cultures showed very high, high, low and very low/null activity. Non-albicans cultures, in terms of their different species, had variability in their activity. In conclusion, the identification of C. albicans and non-albicans was successfully carried out using the CHROMagar and Vitek 2 methods, with consistent results and no significant differences between both methods. The activity of phospholipases and hemolysin varied between the identified species, being mostly high or very high in C. albicans cultures, while in non-albicans this activity showed greater variability.