Maestría en Laboratorio Clínico Mención Microbiología Clínica

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/42733

Browse

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Método automatizado y manual en la detección de escherichia coli productora de betalactamasas de espectro extendido en infección del tracto urinario, en una población de 18 a 35 años de edad.
    (Universidad Técnica de Ambato/Facultad de Ciencias de la Salud/Centro de posgrados, 2024-09-01) Lascano Ortiz, Yadira Michelle Licenciada; Moina Veloz, Álvaro PaulMédico Especialista.
    Urinary Tract Infections (UTIs) are defined as the presence of microorganisms (typically bacteria) in the urinary tract, with or without symptoms. The most common pathogen causing UTIs is Escherichia coli (E. coli), responsible for 75 to 80% of cases. Extended-Spectrum Beta-Lactamases (ESBLs) are plasmid-mediated enzymes frequently produced by E. coli and Klebsiella pneumoniae, which confer resistance to beta-lactam antibiotics, with the exception of cephamycins and carbapenems, as well as other commonly used antibiotics such as aminoglycosides, tetracyclines, trimethoprim/sulfamethoxazole, and quinolones. To compare the automated method and the manual method for detecting ESBL-producing Escherichia coli in urinary tract infections in a population aged 18 to 35 years. Methodology: This was a cross sectional, descriptive, observational study with a qualitative-quantitative approach. Data collection took place in Ambato city, La Merced parish, at the "Divino Niño" clinical laboratory. Urine samples were collected from patients aged 18 to 35 years who presented with UTIs caused by E. coli. ESBL production was determined using the disk diffusion method (manual method) and the Vitek 2 Compact automated system (automated method). Results: Out of 182 samples analyzed, 31 strains suspected of being ESBL-producing E. coli were identified and confirmed by both the manual and automated methods. The manual method confirmed 28 ESBL-positive E. coli strains, while the automated method reported 30 ESBL-positive strains. Both methods were valid and complementary, significantly contributing to clinical management and control of ESBL-producing E. coli infections in the studied population. The choice between methods may depend on resource availability and diagnostic urgency, as both methods offer complementary benefits for clinical management.