Laboratorio Clínico

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/817

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    ACTIVIDAD ANTIFÚNGICA DE ACEITES ESENCIALES EN CANDIDA ALBICANS ATCC 10231
    (2025-02-25) Trujillo Ruiz, John Michael; Proaño Pérez, María Elizabeth
    Candida albicans is a yeast located in the mouth, vagina, intestine, throat, and skin, which can proliferate and generate infections when people have a weakened immune system (1). This yeast generates biofilms, giving it a virulence mechanism and resistance to drugs such as amphotericin B or fluconazole (2)(3)(4). To address this problem, the use of essential oils with antimicrobial properties has been chosen, enhancing this effect through microencapsulation to protect the functional and biological characteristics (5)(6). This research study rigorously examined the remarkable antifungal properties of essential oils derived from Rosmarinus officinalis (rosemary), Ocimum basilicum (basil), and Cymbopogon citratus (lemongrass) against Candida albicans ATCC 10231. The Kirby-Bauer technique was modified according to the CLSI M02 standard. (7). Antifungal activity of essential oils was observed at concentrations up to 25 mg/mL for Ocimum basilicum and Cymbopogon citratus. In comparison, Rosmarinus officinalis showed activity at concentrations up to 50 mg/mL. The antibiofilm activity of Cymbopogon citratus essential oil was assessed using crystal violet dye staining, demonstrating significant inhibition from a concentration of 50 mg/mL to 12.5 mg/mL. The statistical analysis was conducted using an ANOVA test and Tukey's and Bonferroni's multiple comparison analyses. The findings indicated that the essential oils inhibit the growth and development of biofilm in Candida albicans ATCC 10231.
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    EVALUACIÓN DE LA ACTIVIDAD ANTIBIOFILM DE ACEITES ESENCIALES FRENTE A CEPAS ATCC BACTERIANAS
    (2025-02-24) Maldonado Freire, Sofía Antonella; Vicalcundo Córdova, Mario Fernando; Universidad Técnica de Ambato/ Facultad de Ciencias de Salud /Carrera de Laboratorio Clínico
    The increase in multidrug-resistant (MDR) microorganisms and the formation of biofilms as a pathogenicity mechanism represent serious challenges to public health, increasing morbidity and mortality in healthcare-associated infections (HAIs). To address this issue, the present study evaluated the antibiofilm activity of essential oils from Rosmarinus officinalis, Cymbopogon citratus, and Anethum graveolens against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853. The results showed that the essential oils significantly inhibited biofilm formation, achieving efficacy rates of up to 99.74% in S. aureus, 92.34% in E. coli, and limited activity against P. aeruginosa, which exhibited greater resistance due to its complex cellular structure. Notably, C. citratus proved to be the most effective against all strains analyzed, followed by A. graveolens and R. officinalis. These findings highlight the potential of ethanolic extracts as antibiofilm agents by interfering with initial bacterial adhesion and quorum sensing communication systems. Therefore, this research reinforces the therapeutic value of essential oils as a promising alternative to combat antimicrobial resistance, providing a scientific basis for future vivo applications.