Carrera de Biotecnología

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34800

Browse

Subject: search.filters.subject.INDUSTRIA AVÍCOLA

Search Results

Now showing 1 - 4 of 4
  • No Thumbnail Available
    Item
    Análisis genómico de los bacteriófagos líticos f6 y f14 de Salmonella enterica Serovar Infantis
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Gavilanes Tiban, Joselyn Micaela; Calero Cáceres, William Ricardo
    Food contamination by Salmonella represents an emerging global public health crisis, with poultry farms being the principal reservoirs of this pathogen. The excessive use of antibiotics on these farms contributes to the increase in antibiotic resistance. In response to this situation, lytic bacteriophages emerge as a promising alternative for the biocontrol of Salmonella. However, it is essential to perform a detailed analysis of the complete genome of these bacteriophages before their implementation, in order to identify the presence of virulence and antibiotic resistance genes that could affect the safety of their in vivo implementation. In this study, two lytic bacteriophages of S. enterica serovar Infantis, named F6_melonhead and F14_guaytamphage, were sequenced using third-generation Oxford Nanopore technology. Genomic characterization revealed that F6 has a length of 29,097 bp and a GC content of 48.57 percent, encoding 30 functional proteins, while F14 has a length of 59,339 bp, a GC content of 56.44 percent, and encodes 49 functional proteins. In both bacteriophages, the presence of genes producing toxins, bacterial virulence factors, or antibiotic resistance genes was ruled out. Genetic comparison and phylogenetic analysis placed F6_melonhead and F14_guaytamphage within the Berlinvirus and Chivirus genera, respectively. These findings position F6_melonhead and F14_guaytamphage as promising candidates for the biocontrol of Salmonella in the poultry sector in Ecuador.
  • No Thumbnail Available
    Item
    Análisis genómico de los bacteriófagos líticos f6 y f14 de Salmonella enterica Serovar Infantis
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Altamirano Cisneros, Karen Analía; Calero Cáceres, William Ricardo
    with poultry farms being the principal reservoirs of this pathogen. The excessive use of antibiotics on these farms contributes to the increase in antibiotic resistance. In response to this situation, lytic bacteriophages emerge as a promising alternative for the biocontrol of Salmonella. However, it is essential to perform a detailed analysis of the complete genome of these bacteriophages before their implementation, in order to identify the presence of virulence and antibiotic resistance genes that could affect the safety of their in vivo implementation. In this study, two lytic bacteriophages of S. enterica serovar Infantis, named F6_melonhead and F14_guaytamphage, were sequenced using third-generation Oxford Nanopore technology. Genomic characterization revealed that F6 has a length of 29,097 bp and a GC content of 48.57 percent, encoding 30 functional proteins, while F14 has a length of 59,339 bp, a GC content of 56.44 percent, and encodes 49 functional proteins. In both bacteriophages, the presence of genes producing toxins, bacterial virulence factors, or antibiotic resistance genes was ruled out. Genetic comparison and phylogenetic analysis placed F6_melonhead and F14_guaytamphage within the Berlinvirus and Chivirus genera, respectively. These findings position F6_melonhead and F14_guaytamphage as promising candidates for the biocontrol of Salmonella in the poultry sector in Ecuador.
  • No Thumbnail Available
    Item
    Análisis de la influencia de la calidad de agua sobre la microbiota intestinal presente en las heces de pollos de engorde de la empresa Grupo Casa Grande
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Manzaba Yépez, Angela Nicole; Garcés Moncayo, María Daniela
    The gut microbiota includes commensal, symbiotic, and pathogenic microorganisms that inhabit complex multicellular organisms. Among the main functions, the following stand out: regulation of metabolic activities, absorption of nutrients and prevention of colonization by pathogens. However, the microbiota can be altered by various factors such as water, food, antibiotic intake and geographical location. In this study, it was known whether water quality influences the intestinal microbiota of Cobb 500 broiler chickens from two farms (San José and Las Orquídeas) belonging to the Grupo Casa Grande company. For this purpose, a metagenomic amplicon analysis of the 16s rRNA gene was carried out, using DNA extracted from 4 fecal samples of broiler chickens. Data processing was performed on the Galaxy platform linked to QIIME2. The analysis of water quality was within the limits established by INEN 1108 standards and the Cobb 500 Chicken Management Manual, with the exception of water hardness (Las Orquídeas farm). The Shannon index and PCoA revealed that samples from both farms present a high microbial diversity of OTUs and a minimal difference in the composition of the microbial community. The analyzes of relative abundance, differential abundance and alpha rarefaction showed that the most abundant and richest taxa of the fecal microbiota were Lactobacillus sp and Romboutsia sp. The water quality of the farms did not influence the composition of the intestinal microbiota of the chickens.
  • No Thumbnail Available
    Item
    Análisis de la influencia de la calidad de agua sobre la microbiota intestinal presente en las heces de pollos de engorde de la empresa Grupo Casa Grande
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Aguilar Castillo, Carla Melanie; Garcés Moncayo, María Daniela
    The gut microbiota includes commensal, symbiotic, and pathogenic microorganisms that inhabit complex multicellular organisms. Among the main functions, the following stand out: regulation of metabolic activities, absorption of nutrients and prevention of colonization by pathogens. However, the microbiota can be altered by various factors such as water, food, antibiotic intake and geographical location. In this study, it was known whether water quality influences the intestinal microbiota of Cobb 500 broiler chickens from two farms (San José and Las Orquídeas) belonging to the Grupo Casa Grande company. For this purpose, a metagenomic amplicon analysis of the 16s rRNA gene was carried out, using DNA extracted from 4 fecal samples of broiler chickens. Data processing was performed on the Galaxy platform linked to QIIME2. The analysis of water quality was within the limits established by INEN 1108 standards and the Cobb 500 Chicken Management Manual, with the exception of water hardness (Las Orquídeas farm). The Shannon index and PCoA revealed that samples from both farms present a high microbial diversity of OTUs and a minimal difference in the composition of the microbial community. The analyzes of relative abundance, differential abundance and alpha rarefaction showed that the most abundant and richest taxa of the fecal microbiota were Lactobacillus sp and Romboutsia sp. The water quality of the farms did not influence the composition of the intestinal microbiota of the chickens.