Carrera de Biotecnología

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Subject: search.filters.subject.CULTIVO IN VITRO

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    Optimización del protocolo para la fase de introducción in vitro de la flor del Ranúnculo Persa (Ranunculus asiaticus) en la empresa LEPLANTEC S.A.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Paredes Pico, Ariana Paulina; De la Torre Olvera, Helena Maritza
    The optimization of the protocol for the in vitro introduction phase of the Persian Buttercup (R. asiaticus) was conducted in collaboration with LEPLANTEC S.A., where the first stage of in vitro plant micropropagation was analyzed due to its high endogenous and exogenous contamination. The objective was to improve the standardized culture medium protocol through combined treatments. Bulbs of the flower were collected to evaluate two concentrations of sodium hypochlorite, determining that the 2% concentration presented a low percentage of shoots with total oxidation, although without significant difference from the 1.5% concentration. Antibiotics were directly applied to the shoots during planting, with gentamicin showing the lowest incidence percentage. The effectiveness of Plant Preservative Mixture as a bacteriostatic agent was measured, with the 1 ml concentration being the most effective. Finally, the best concentration of the hormones BAP and ANA was determined, with the 1 ml concentration being optimal as it promoted adequate shoot growth in this initial stage. The combined treatment D2P1A1H2 was the most effective, identified through a factorial design, showing the highest percentage of shoot viability, achieving a high degree of viability and proper development of the Persian Buttercup shoots. This research allowed the identification and optimization of the critical components of the in vitro micropropagation protocol for the Persian Buttercup, significantly improving the process efficiency and establishing a more robust protocol for the investigative purposes of LEPLANTEC S.A.
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    Optimización del proceso de enraizamiento in vitro de arándano (Vaccinium corymbosum L.) cultivar Biloxi
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Tubón Pilatasi, Yadira Michelle; Sánchez Garnica, Manoella Alejandra
    Blueberries have gained popularity owing to their health benefits. Ecuador, with more hours of sunlight and the absence of time zones, offers a favorable environment for enhancing and expanding these crops. However, there are significant obstacles to the limited and expensive availability of plant materials. This problem arises because conventional propagation cannot cope with the demand for plants owing to the difficult rooting of explants caused by physical and physiological stress. In this context, plant micropropagation is a viable solution. This technique allows large-scale seedlings to be obtained from cuttings, thereby overcoming the limitations of conventional propagation. Therefore, this research aims to optimize the in vitro rooting process of blueberry (Vaccinium corymbosum L.) Biloxi variety. To achieve this, we will evaluate disinfection treatments, culture media, and phytohormone levels. The objective was to facilitate ex vitro establishment and meet the growing demand for plants. The best disinfection treatment was 2 percent sodium hypochlorite (NaOCl) for 15 min. Under these conditions, 9 percent contamination, 91 percent viability, and 7 percent oxidation were observed. In addition, treatments with WPM yielded better results, with an average of eight leaves and a shoot length of 1,409 cm. In addition, 84 percent rooting was evident, with an average root length of 3.24 cm and 11.76 roots per explant using 1.5 mg per L of the phytohormone AIB.
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    Optimización del proceso de enraizamiento in vitro de arándano (Vaccinium corymbosum L.) cultivar Biloxi
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Guamán Chito, Mary Gissela; Sánchez Garnica, Manoella Alejandra
    Blueberries have gained popularity owing to their health benefits. Ecuador, with more hours of sunlight and the absence of time zones, offers a favorable environment for enhancing and expanding these crops. However, there are significant obstacles to the limited and expensive availability of plant materials. This problem arises because conventional propagation cannot cope with the demand for plants owing to the difficult rooting of explants caused by physical and physiological stress. In this context, plant micropropagation is a viable solution. This technique allows large-scale seedlings to be obtained from cuttings, thereby overcoming the limitations of conventional propagation. Therefore, this research aims to optimize the in vitro rooting process of blueberry (Vaccinium corymbosum L.) Biloxi variety. To achieve this, we will evaluate disinfection treatments, culture media, and phytohormone levels. The objective was to facilitate ex vitro establishment and meet the growing demand for plants. The best disinfection treatment was 2 percent sodium hypochlorite (NaOCl) for 15 min. Under these conditions, 9 percent contamination, 91 percent viability, and 7 percent oxidation were observed. In addition, treatments with WPM yielded better results, with an average of eight leaves and a shoot length of 1,409 cm. In addition, 84 percent rooting was evident, with an average root length of 3.24 cm and 11.76 roots per explant using 1.5 mg per L of the phytohormone AIB.