Carrera de Biotecnología

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Subject: search.filters.subject.ANTIBIÓTICOS

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    Eficiencia del almidón de chonta (Bactris gasipaes) como agente encapsulante sobre la actividad antimicrobiana de la doxiciclina
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Aponte Urrutia, Kerly Anais; López Hernández, Orestes Darío
    Doxycycline is an antibiotic of great interest in the health area, since it serves as a treatment for a variety of bacterial infections, but it has a drawback since it tends to degrade very easily at the time of its production, that is why new alternatives have been sought to preserve its bioactive components and protect it from contamination and even increase its bioavailability with the help of microencapsulation which is a technique that uses encapsulating materials such as natural polymers in order to generate greater effectiveness in encapsulation. For this, three suspensions were made with different proportions, using analysis techniques and verification of microencapsulation efficiency which were, performance analysis, statistical analysis, thermograms by means of differential scanning calorimetry (DSC) and antimicrobial evaluation, which was carried out by means of antibiograms of diffusion disks in Müller Hinton agar, to determine the inhibition of the microencapsulate against the E. coli bacteria. Through the different analyses carried out, it was obtained that the best performance and inhibition halos were given in the proportion 20:80, in addition to this, through the thermograms it was possible to observe the melting peaks where it was also represented that the best curve was that of the proportion 20:80. This is thanks to the fact that with a higher polymeric load, the percentage of microencapsulation efficiency increases. This could also be observed with the help of an-ANOVA analysis, which allowed the most significant performance to be seen through statistical graphs.
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    Caracterización de los perfiles de resistencia antimicrobiana de Escherichia coli aislada de mamíferos en cautiverio del EcoZoológico San Martín – Baños de Agua Santa
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2022-09) Medina Pujos, Anabell Yessenia; Calero Cáceres, William Ricardo
    Antimicrobial resistance (AMR) has become a public health problem around the world; therefore, to prevent the emergence and spread of pathogenic bacteria, measures should be taken by adopting a One Health perspective. Around the world, one of the factors for the emergence of resistant pathogens has been the zoonotic exchange between animals and humans, and its association with the use of different antimicrobials in human and veterinary medicine. For this reason, the study of wild or captive species is essential. This research focuses on characterizing antimicrobial resistance profiles in E. coli isolates from captive mammals at the San Martín Eco Zoo in Baños de Agua Santa. From a total of 27 faecal samples collected, 90 strains were isolated. 32 strains presented resistance to more than three families of antibiotics; however, only strains E57 and E75 isolated from ocelots (Leopardus pardalis) showed resistance to six families of antibiotics. The antibiotics with the highest percentage of resistance were ampicillin (97.5), ceftriaxone (95), cefuroxime (95), trimethoprim/sulfamethoxazole (52.5), ceftazidime (47.5) and ciprofloxacin (37.5). The generated profiles showed the relationship between resistance to antimicrobials and the diet of the mammals studied, observing that those isolated from mammals with a meat-based diet were multi-resistant E. coli; In addition, the antibiotic treatment received helped with the presence of more resistant isolates. The results obtained indicate that captive species can be potential reservoirs of multi-resistant bacteria to antibiotics.