Carrera de Biotecnología

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    Evaluación de la producción y conservación de bacteriófagos líticos de Salmonella enterica con medios de cultivo económicos y sustancias crioprotectoras alternativas
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Romero Rovayo, Nathaly Johanna; Calero Cáceres, William Ricardo
    Bacteriophages represent an alternative for the elimination of potentially pathogenic microorganisms. However, their use involves numerous stages that include selection, generation of high phage titers, preservation, characterization, and assessments of their effectiveness. These processes require a significant financial investment in culture media and preservation methods, which could limit their applicability in scaling up. Therefore, in this study, cost-effective culture media were generated and alternative cryoprotective substances were used to improve the efficiency in obtaining and preserving high phage titers, thus facilitating their future industrial use. The formulation of culture media was carried out, using the avian-origin strain S. enterica serovar Infantis S11V23 as the host, and the bacteriophage Berlinvirus sp. F6_melonhead. Initially, the behavior of the bacteria and the phage against each formulation was analyzed. Finally, alternative cryoprotective substances were employed to evaluate the preservation of the titer over a period of 56 days at temperatures of 4, 20, and -70 degrees Celsius. The feasibility of obtaining bacterial and phage biomass using cost-effective culture media based on malt extract and brewer's yeast extract was proven, being effective for generating high phage titers. Furthermore, the stability of the bacteriophages at -70 Degrees Celsius was achieved using de-lactosed milk as a cryoprotective substance. The results highlight the possibility of scaling up bacteriophage production with limited resources and greater efficiency in generating bioproducts for the control of S. enterica useful for the poultry industry.
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    Bioprospección de bacteriófagos líticos de Salmonella enterica multirresistente de origen avícola de la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Topa Pila, Paulina Liseth; Calero Cáceres, William Ricardo
    The use of bacteriophages has emerged as a promising alternative to address the antimicrobial resistance crisis, driven by the indiscriminate use of antibiotics in the poultry industry. This study isolated lytic bacteriophages specific to multi-resistant S. enterica of poultry origin in Tungurahua province from contaminated water sources and evaluated them based on their lytic profiles and low-temperature stability. Under the Microbiology and Biotechnology research line, the isolation and purification of bacteriophages was carried out using two strains of S. enterica serovar Infantis, by the double-layer agar method. Lytic profiles were evaluated by the gout test. The stability of the bacteriophages was tested through the reduction of the phage titre, at refrigeration and freezing temperatures, with pH 5.5 and 7.2. We isolated a total of 27 bacteriophages. Evaluation against 38 Salmonella isolates from poultry and five different S. enterica serovars revealed seven bacteriophages with a broad host range. Stability tests of bacteriophages F6, F7M1, and F4M1B, showed that temperature and pH significantly influenced phage titer reduction and bacterial lysis, underscoring the importance of understanding bacteriophages stability conditions for successful application. The results highlight the potential use of these lytic bacteriophages in designing biocontrol products for the poultry industry.
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    Bioprospección de bacteriófagos líticos de Salmonella enterica multirresistente de origen avícola de la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Morales Cunalata, Katheryne Yolanda; Calero Cáceres, William Ricardo
    The use of bacteriophages has emerged as a promising alternative to address the antimicrobial resistance crisis, driven by the indiscriminate use of antibiotics in the poultry industry. This study isolated lytic bacteriophages specific to multi-resistant S. enterica of poultry origin in Tungurahua province from contaminated water sources and evaluated them based on their lytic profiles and low-temperature stability. Under the Microbiology and Biotechnology research line, the isolation and purification of bacteriophages was carried out using two strains of S. enterica serovar Infantis, by the double-layer agar method. Lytic profiles were evaluated by the gout test. The stability of the bacteriophages was tested through the reduction of the phage titre, at refrigeration and freezing temperatures, with pH 5.5 and 7.2. We isolated a total of 27 bacteriophages. Evaluation against 38 Salmonella isolates from poultry and five different S. enterica serovars revealed seven bacteriophages with a broad host range. Stability tests of bacteriophages F6, F7M1, and F4M1B, showed that temperature and pH significantly influenced phage titer reduction and bacterial lysis, underscoring the importance of understanding bacteriophages stability conditions for successful application. The results highlight the potential use of these lytic bacteriophages in designing biocontrol products for the poultry industry.