Carrera de Biotecnología

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Author: search.filters.author.Paredes Pico, Ariana Paulina

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    Optimización del protocolo para la fase de introducción in vitro de la flor del Ranúnculo Persa (Ranunculus asiaticus) en la empresa LEPLANTEC S.A.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Paredes Pico, Ariana Paulina; De la Torre Olvera, Helena Maritza
    The optimization of the protocol for the in vitro introduction phase of the Persian Buttercup (R. asiaticus) was conducted in collaboration with LEPLANTEC S.A., where the first stage of in vitro plant micropropagation was analyzed due to its high endogenous and exogenous contamination. The objective was to improve the standardized culture medium protocol through combined treatments. Bulbs of the flower were collected to evaluate two concentrations of sodium hypochlorite, determining that the 2% concentration presented a low percentage of shoots with total oxidation, although without significant difference from the 1.5% concentration. Antibiotics were directly applied to the shoots during planting, with gentamicin showing the lowest incidence percentage. The effectiveness of Plant Preservative Mixture as a bacteriostatic agent was measured, with the 1 ml concentration being the most effective. Finally, the best concentration of the hormones BAP and ANA was determined, with the 1 ml concentration being optimal as it promoted adequate shoot growth in this initial stage. The combined treatment D2P1A1H2 was the most effective, identified through a factorial design, showing the highest percentage of shoot viability, achieving a high degree of viability and proper development of the Persian Buttercup shoots. This research allowed the identification and optimization of the critical components of the in vitro micropropagation protocol for the Persian Buttercup, significantly improving the process efficiency and establishing a more robust protocol for the investigative purposes of LEPLANTEC S.A.