Carrera de Biotecnología

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Author: search.filters.author.Nuñez Ambato, Erika FernandaSubject: search.filters.subject.ACTIVIDAD ANTIINFLAMATORIA

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    Evaluación de la vida útil de un extracto obtenido de la cáscara de piña (Ananas comosus) con propiedades antioxidantes y antiinflamatorias
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Nuñez Ambato, Erika Fernanda; Fernández Rivero, Danae
    Considering the growing interest in the utilization of agricultural waste, pineapple peel stands out as a rich source of bioactive compounds. The need to replace synthetic antioxidants and anti-inflammatories, associated with health risks, emphasizes the importance of exploring natural sources. Additionally, the assessment of shelf life is crucial to ensure the stability and efficacy of the extract over time. The extraction process began using ethanol and distilled water as solvents in different proportions. The concentration of polyphenols was determined using the Folin-Ciocalteu method, demonstrating that the ethanol treatment was the most efficient. Total flavonoid evaluation was performed on the extract with the highest polyphenol concentration using the colorimetric method of aluminum chloride. Furthermore, the analysis of antioxidant activity was conducted through the DPPH assay, resulting in an inhibition percentage of 52.27 percent. The analysis of anti-inflammatory activity was also carried out using the egg albumin denaturation assay, revealing significant percentages of denaturation inhibition, reaching 73.15 percent. Stability was analyzed over a month and showed a slight increase in pH and variation in humidity, confirming the robustness of the extract. This study provides valuable information on the shelf life of pineapple peel extract, highlighting its potential as a natural source of antioxidants and anti-inflammatories. This contributes to the expansion of knowledge on the utilization of agricultural by-products and the search for natural solutions to health challenges.