Carrera de Biotecnología

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Author: search.filters.author.Lalaleo Camino, Christian JosuéSubject: search.filters.subject.MICROALGAS

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    Caracterización de la producción de metabolitos secundarios por microorganismos fotosintéticos
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Lalaleo Camino, Christian Josué; García Solís, Mario Daniel
    Global population growth has driven demand for food resources, causing a negative environmental impact through the expansion of agriculture and livestock farming. In response, photosynthetic microorganisms have been highlighted as an alternative source of bioactive compounds, such as proteins, carbohydrates, and pigments. This project evaluated the impact of carbon and nitrogen on the growth kinetics, production of proteins, carbohydrates, and pigments of the CLMR1 isolate, as well as its influence on cell morphology. Autotrophic and mixotrophic cultures were carried out, using carbon dioxide with two organic carbon sources (ethylene glycol and sodium acetate) and different concentrations of sodium nitrate (0, 0.625, 1.25 and 2.5) grams per liter. The most outstanding results were observed in the culture with 2.5 grams per liter of sodium nitrate and ethylene glycol, achieving the highest cell concentration (21,000,000 cells per milliliter), maximum amount of carbohydrates (1015.78 micrograms per milliliter) and the highest synthesis of chlorophyll. and carotenoids (0.274 and 0.207 micrograms per milliliter). Additionally, treatment with 2.5 grams per liter of sodium nitrate and sodium acetate produced the highest protein concentration (2.31 micrograms per milliliter). However, cultures without sodium nitrate showed the lowest synthesis of each metabolite and the minimum biomass production (200,000 cells per milliliter). Morphological changes were evident in the cells, highlighting the importance of optimal culture conditions for the synthesis of metabolites and their application in the sustainable production of high-value biomolecules.