Carrera de Biotecnología
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Item Evaluación de la vida útil de un extracto con propiedades antioxidantes obtenido de hojas de arándano (Vaccinium corymbosum)(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Aguilar Sisalema, Jennifer Victoria; Fernández Rivero, DanaeInterest in the use of natural products has increased in recent years due to the therapeutics proprieties they can have on health, one of them being antioxidant activity. Among these products are extracts that can be made from plant waste; however, to ensure their quality, it is necessary to determine their shelf life. One of waste with antioxidants of natural origin, specifically phenolic compounds, are blueberry (Vaccinium corymbosum) leaves. Ultrasound-assisted extraction was used to obtain the extract, where the use of a 70 percent ethanol concentration, a ratio of 1 gram of plant matter to 10 milliliters of solvent and a temperature of 50 degrees Celsius made it possible to obtain a higher concentration of phenolic compounds with a value of 136,85 milligrams of gallic acid per gram of dry weight. Subsequently, its antioxidant activity was determined using DPPH method, where an inhibition of 80,78 percent was recorded, while in Trolox equivalents it was 476,93 micromoles per gram of dry weight, which is considered as a high antioxidant activity of this plant species. Finally, the shelf life on the extract when stored in amber glass jars at a refrigeration temperature ranging from 2 to 8 degrees Celsius was found to be 2,02 weeks, following first order kinetic modelling with a rate constant of 0,0413 per week.