Maestría de Medicina Veterinaria
Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/43039
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Item Presencia de genes de resistencia a los antibióticos en Escherichia coli aislada de carne de pollo que se expende en el cantón de Ambato(2024-11) Gómez Aillòn Diego José; Cruz Quintana Sandra MargaritaAntimicrobial resistance is currently a public health problem in Ecuador. It seems that the indiscriminate use of antibiotics in animal production is a way for the development of this resistance. E. coli is a pathogen that affects animals and people and has developed resistance to several antibiotics in recent years. Cephalosporins and quinolones are used in broiler production and are two families of antibiotics to which strains of E. coli They have shown resistance. This resistance is mainly mediated by resistance genes such as gyrA and blaCTXM. Studies have searched for these mechanisms in E. coli strains from chicken meat, as it is one of the main meats consumed in Ecuador. The present study aimed to evaluate the antibioresistance and the presence of resistance genes of 31 Escherichia coli strains to antibiotics. Antibioresistance was carried out by the agar diffusion method and the search for resistance genes through PCR. In this way, a Kruskall Wallis test and the Mann Whitney U test were performed for the antibioresistance data, and for the genes, prevalence and a chi-square test were analyzed, with a 95% confidence interval. The results for the antibioresistance of the strains were resistant to ciprofloxacin 45.1% and ceftriaxone 25.8% and the antibiotics that provided greater susceptibility were gentamicin and amikacin with a p≤0.05. For the gyrA genes of CIP and blaCTXM of CX there were prevalence of 100% and 96.77% respectively. When comparing the difference between the origin of the sales center and the resistance gene, there was no statistical difference p≥ 0.05 in both genes. Finding resistance to antibiotics gives us a warning that the indiscriminate use of these in the country's animal production could be an important factor for this resistance. The prevalence of genes is a guide to the main resistance mechanism that could be used by E. coli, such as mutations in genes and genes that encode enzymes that provide resistance. And finally, finding antibioresistance and resistance genes in chicken meat in the Ambato canton is a warning of the possible risk of transmissibility of resistant strains that could generate resistance to antibiotics in people. However, the current data in Ecuador on this possibility is not yet being studied with the importance of the case. Therefore, studies like this open a gap for us to analyze everything that antibioresistance implies and the repercussions on public health in Ecuador.