Evaluación de la eficacia in vitro del aceite esencial de romero (Rosmarinus officinalis) como control natural de pulgas en caninos domésticos (Canis lupus familiaris)

Abstract

In the field of human health, pest control has always been very important both in the care of crops and in the fact that they become one of the main factors of disease transmission, with the flea becoming an important vector for the transmission and how to trigger many diseases. Due to this problem, a countless number of insecticides have been created, which due to constant and misuse have created fleas resistant to them, for this reason, this research seeks to evaluate the in vitro effectiveness of rosemary essential oil (Rosmarinus officinalis ) as a natural control of fleas in domestic canines (Canis lupus familiaris). 90 fleas were collected using the fine comb technique, which were subjected to different rosemary treatments (1%, 2%, 4%, 8%) as well as a positive control (Fipronil) and a negative control (distilled water). The fleas were identified as Ctenocephalides felis, based on the morphological characteristics of the head and antennae. In relation to the effectiveness of rosemary oil, it was found that the mortality rate increased with increasing concentration. The highest mortality rate was obtained when rosemary essential oil was used at a concentration of 8%, reaching 50% after 1 hour of application and after 24 and 48 hours mortality reached 100%. Based on the results, it is suggested to carry out in vivo effectiveness evaluations to determine its safety on dogs.