Obtención del péptido antimicrobiano aedesina por medio de un sistema de autoescisión mediado por inteina, utilizando un sistema de expresión en Escherichia coli BL21 (DE3)

Abstract

Antibiotics have significantly improved people's lifestyle since their implementation. However, at present, the indiscriminate use of these drugs has generated a health crisis due to antimicrobial resistance. The current trend shows an increase in the number of resistant strains that cannot be treated with conventional antibiotics, which has led to the need to develop a new generation of antibiotics. One of the most promising alternatives is the application of antimicrobial peptides, among which aedesin stands out. The present research project focuses on the implementation of a purification strategy for aedesin. This strategy consists of an intein-mediated self-cleavable system, which possesses a prohibition condition that disables self-cleavage in the presence of high salt concentrations. A heterologous system of synthesis in E. coli BL21 was used for expression. In conclusion, synthesis in bacteria offers several advantages compared to other methods of obtaining peptides. Furthermore, the strategy used for the purification of aedesin has proven to be effective. However, there are still challenges to be overcome, such as peptide toxicity and basal expression. Despite these drawbacks, the successful expression and purification of aedesin position it as an interesting candidate for analysis in future clinical studies.