Ciencia e Ingeniería en Alimentos y Biotecnología
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Item Estudio de la contaminación por la presencia de partículas biológicas aerotransportables en el relleno sanitario de la Mancomunidad GIDS Pujilí - Saquisilí(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-12) Flores Silva, Valeria Estefanía; Córdova Suárez, Manolo AlexanderThe aim of research was to study Study of pollution by the presence of airborne biological particles from the landfill the Commonwealth of GIDS Pujilí-Saquisilí. The sampling taked place in two points, at different times (09h00 and 12h00) and during 10 working days, in situ was registered conditions of temperature and relative humidity. Through air inoculation in the Brain Heart Infusion medium, the microbial concentration in UFC/ml was determined, the analysis showed that concentrations were below the level allowed by International proposals, it attributing the higher concentration to the treatments; a1b1c5 with a value of 22.08 UFC/ml at 17 Celsius degrees and 56 percent RH in the commons waste area and a1b2c2 with a value of 22.32 UFC/ml at 18 Celsius degrees and 52 percent RH in the hospital waste area. From the 40 samples of air taken, 64 bacteria were isolated. That with IMVIC biochemical tests were identified, this analysis showed species with enteric characteristics, such as: Enterococcus faecalis or Shigella flexneri, Salmonella or Citrobacter freundii, Pantoea agglomerans, Enterobacter cloacae type II, Staphylococcus aureus, Klebsiella pneumoniae or Enterobacter, Escherichia coli, Citrobacter Koseri and Klebsiella oxytoca, Moreover, the biological risk to which the workers of the filling were exposed was evalued, this evaluation was based on Royal Decree 664/1997. It was established that air of the landfill is composed of biological agents belonging to groups 2 and 3. This study shows that exists moderate pollution in the facilitiesItem Desarrollo de instructivos de Seguridad e Higiene Industrial a partir del análisis aerobiológico del relleno sanitario de la Empresa Pública Municipal Gestión Integral de Desechos Sólidos del cantón Salcedo(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-05) Vásconez Hurtado, Ingrid Nicole; Córdova Suárez, Manolo AlexanderThe aerobiological study was made in the Sanitary Landfill of Salcedo’s city, which process consisted of taking the air with sterile syringes of 20 cm3 and injecting it into 10 ml of Brain-heart infusion medium to incubate the microorganisms at 37°C. The objective was study the proliferation of microorganisms within the human organism, this study was carried out in the solid waste disposal area and the hospital waste area, where the presence of pathogenic microorganisms which may be responsible for infections in the worker was evaluated. To determine the biological risk was taken as reference the Royal Decree 664/1997 which establishes the microorganism’s classification according to their infectious capacity. In this research was found a couple of dangerous bacteria such as Escherichia coli and Salmonella typhi, which belong to the third group of this classification, indicating that these microorganisms are capable to produce serious pathologies and are therefore a serious risk to the worker's health and a possibility of spreading into the community. Due to the present risk in the evaluated area, a biosecurity instructive and an instructive about the use of PPE (personal protection equipment) and occupational hygiene were made, which mitigate the risk due to exposure to Salcedo Municipal GAD workers.Item Desarrollo de instructivos de Seguridad e Higiene Industrial a partir del análisis aerobiológico del relleno sanitario de la Empresa Pública Municipal Gestión Integral de Desechos Sólidos del cantón Salcedo(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-05) Solís Sánchez, Diego Alexis; Córdova Suárez, Manolo AlexanderThe aerobiological study was made in the Sanitary Landfill of Salcedo’s city, which process consisted of taking the air with sterile syringes of 20 cm3 and injecting it into 10 ml of Brain-heart infusion medium to incubate the microorganisms at 37°C. The objective was study the proliferation of microorganisms within the human organism, this study was carried out in the solid waste disposal area and the hospital waste area, where the presence of pathogenic microorganisms which may be responsible for infections in the worker was evaluated. To determine the biological risk was taken as reference the Royal Decree 664/1997 which establishes the microorganism’s classification according to their infectious capacity. In this research was found a couple of dangerous bacteria such as Escherichia coli and Salmonella typhi, which belong to the third group of this classification, indicating that these microorganisms are capable to produce serious pathologies and are therefore a serious risk to the worker's health and a possibility of spreading into the community. Due to the present risk in the evaluated area, a biosecurity instructive and an instructive about the use of PPE (personal protection equipment) and occupational hygiene were made, which mitigate the risk due to exposure to Salcedo Municipal GAD workers.