Ciencia e Ingeniería en Alimentos y Biotecnología

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    Desarrollo de instructivos de Seguridad e Higiene Industrial a partir del análisis aerobiológico del relleno sanitario de la Empresa Pública Municipal Gestión Integral de Desechos Sólidos del cantón Salcedo
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-05) Vásconez Hurtado, Ingrid Nicole; Córdova Suárez, Manolo Alexander
    The aerobiological study was made in the Sanitary Landfill of Salcedo’s city, which process consisted of taking the air with sterile syringes of 20 cm3 and injecting it into 10 ml of Brain-heart infusion medium to incubate the microorganisms at 37°C. The objective was study the proliferation of microorganisms within the human organism, this study was carried out in the solid waste disposal area and the hospital waste area, where the presence of pathogenic microorganisms which may be responsible for infections in the worker was evaluated. To determine the biological risk was taken as reference the Royal Decree 664/1997 which establishes the microorganism’s classification according to their infectious capacity. In this research was found a couple of dangerous bacteria such as Escherichia coli and Salmonella typhi, which belong to the third group of this classification, indicating that these microorganisms are capable to produce serious pathologies and are therefore a serious risk to the worker's health and a possibility of spreading into the community. Due to the present risk in the evaluated area, a biosecurity instructive and an instructive about the use of PPE (personal protection equipment) and occupational hygiene were made, which mitigate the risk due to exposure to Salcedo Municipal GAD workers.
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    Desarrollo de instructivos de Seguridad e Higiene Industrial a partir del análisis aerobiológico del relleno sanitario de la Empresa Pública Municipal Gestión Integral de Desechos Sólidos del cantón Salcedo
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-05) Solís Sánchez, Diego Alexis; Córdova Suárez, Manolo Alexander
    The aerobiological study was made in the Sanitary Landfill of Salcedo’s city, which process consisted of taking the air with sterile syringes of 20 cm3 and injecting it into 10 ml of Brain-heart infusion medium to incubate the microorganisms at 37°C. The objective was study the proliferation of microorganisms within the human organism, this study was carried out in the solid waste disposal area and the hospital waste area, where the presence of pathogenic microorganisms which may be responsible for infections in the worker was evaluated. To determine the biological risk was taken as reference the Royal Decree 664/1997 which establishes the microorganism’s classification according to their infectious capacity. In this research was found a couple of dangerous bacteria such as Escherichia coli and Salmonella typhi, which belong to the third group of this classification, indicating that these microorganisms are capable to produce serious pathologies and are therefore a serious risk to the worker's health and a possibility of spreading into the community. Due to the present risk in the evaluated area, a biosecurity instructive and an instructive about the use of PPE (personal protection equipment) and occupational hygiene were made, which mitigate the risk due to exposure to Salcedo Municipal GAD workers.
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    Evaluación de bioaerosoles asociados en el sitio de desposición final de residuos sólidos en la Empresa Pública de Aseo y Gestión Ambiental del cantón Latacunga (EPAGAL)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-04) Acosta Hurtado, César Ismael; Vargas López, José Homero
    The bioaerosols present in the common waste area and in the hospital waste area of the final disposal site of solid wastes were evaluated in the Empresa Publica de Aseo y Gestion Ambiental of Latacunga canton (EPAGAL). Sampling was performed at five different points, recording environment temperature and relative humidity, for 24 days from Monday to Saturday at 10:00 a.m. and 2:00 p.m. in each of the zones, inoculating 1000 cc of air in breeding grounds Brain Heart Infusion. Microbial concentrations were estimated using a McFarland curve, with results in the range of 1,00135*1010 CFU/ml at 16 °C and 63% RH and 1,06*107 CFU/ml at 13 °C and 100% RH for common waste, while for hospital waste 6,9041*109 CFU / ml at 16 °C and 63% RH and 1,06*107 CFU/ml at 13 °C and 94% RH. By means of the IMVIC biochemical tests, a total of 91 bacteria were isolated, with six species of enterobacteria Shigella sonnei, Citrobacter freundii, Proteus mirabilis, Klebsiella pneumoniae, Citrobacter koseri and Escherichia coli and three genera Salmonella, Enterobacter and Providencia. It was not possible to identify 15 bacterial strains because they did not present bibliographically comparable results. The presence of enterobacteria and high microbial concentrations in these areas are the main cause of gastrointestinal diseases in both workers and surrounding populations.
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    Evaluación de bioaerosoles asociados en el sitio de desposición final de residuos sólidos en la Empresa Pública de Aseo y Gestión Ambiental del cantón Latacunga (EPAGAL)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-04) Muso Jami, Edwin Fabian; Vargas López, José Homero
    The bioaerosols present in the common waste area and in the hospital waste area of the final disposal site of solid wastes were evaluated in the Empresa Publica de Aseo y Gestion Ambiental of Latacunga canton (EPAGAL). Sampling was performed at five different points, recording environment temperature and relative humidity, for 24 days from Monday to Saturday at 10:00 a.m. and 2:00 p.m. in each of the zones, inoculating 1000 cc of air in breeding grounds Brain Heart Infusion. Microbial concentrations were estimated using a McFarland curve, with results in the range of 1,00135*1010 CFU/ml at 16 °C and 63% RH and 1,06*107 CFU/ml at 13 °C and 100% RH for common waste, while for hospital waste 6,9041*109 CFU / ml at 16 °C and 63% RH and 1,06*107 CFU/ml at 13 °C and 94% RH. By means of the IMVIC biochemical tests, a total of 91 bacteria were isolated, with six species of enterobacteria Shigella sonnei, Citrobacter freundii, Proteus mirabilis, Klebsiella pneumoniae, Citrobacter koseri and Escherichia coli and three genera Salmonella, Enterobacter and Providencia. It was not possible to identify 15 bacterial strains because they did not present bibliographically comparable results. The presence of enterobacteria and high microbial concentrations in these areas are the main cause of gastrointestinal diseases in both workers and surrounding populations.