Ciencia e Ingeniería en Alimentos y Biotecnología
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Item Identificación y clasificación de bacterias con potencial en biotecnología vegetal(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2018-12) Reinoso Caicedo, Gladys Estefanía; Rodríguez Meza, Carlos AlbertoForty-four bacteria were classified, which were separated into 3 groups of interest according to their possible uses in plant biotechnology processes. In the phenotypic study of the strains, data were obtained from their macroscopic, microscopic and physiological characteristics. By direct observation of the colony, stains, tests of resistance to different conditions of the medium and tests of use of different sources of carbon. In the molecular study the characteristics were obtained by the BOX PCR reaction of all the strains. Both, phenotypic and molecular characteristics were analyzed using the coefficient of Simple similarity (SSM) and the UPGMA algorithm. The species groups formed in this analysis helped to select the strains that were identified by sequencing the 16s rDNA gene. To obtain the species that belonged each strain was carried out the phylogenetic analysis in the program PHYDIT, using the coefficient of Jukes-Cantor and Neighbour joining algorithm to obtain the phylogenetic tree. From which a total of 16 different species were obtained, where those belonging to the genus Bacillus were the dominant ones with a total of 11 species. Two species of Enterobacter were also obtained and only one species of Rhizobium, Pseudomonas and Lysinibacillus. It should be emphasized that these species are distributed in the 3 groups of interest demonstrating the diversity existing in the collection of bacteria used in this study.