Ciencia e Ingeniería en Alimentos y Biotecnología

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Author: search.filters.author.Paredes Paredes, Jennifer Katheryn

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    Caracterización de la producción de metabolitos primarios y secundarios por Scenedesmus sp. nativa del Ecuador
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Paredes Paredes, Jennifer Katheryn; García Solís, Mario Daniel
    Currently, global problems have arisen, such as the limited availability of food and population growth, causing the depletion of natural resources, emissions of greenhouse gases and nitrous oxide from fertilizers and pesticides, methane production by ruminants, and deforestation. At the same time, the need to feed the growing population has driven the development of techniques to obtain microalgae supplements, since they produce active compounds and metabolites such as carbohydrates, proteins, pigments, vitamins and amino acids. For this reason, this research seeks to evaluate the production of primary and secondary metabolites by the microalga Scenedesmus sp. native to Ecuador, quantifying its capacity to produce proteins, carbohydrates and pigments under different culture conditions. Supplements to the BBM medium with different concentrations of sodium nitrate (0, 1.25, 2.5 and 5 grams per liter) and carbon sources (carbon dioxide, ethylene glycol, sodium acetate) were used. These conditions were combined to obtain 12 treatments. The culture scenario was 21 degrees Celsius, a light-dark cycle of 16 and 8 hours, and constant aeration of 200 milliliters per minute with 0.04 percent volume-volume carbon dioxide. The results showed that the best treatment was the mixotrophic culture with ethylene glycol and high concentration of sodium nitrate, producing the highest amount of proteins, carbohydrates and pigments (chlorophyll and carotenoids). It was also determined that the growth kinetics of Scenedesmus sp. varies according to the concentration of sodium nitrate in the medium.