Ciencia e Ingeniería en Alimentos y Biotecnología

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Author: search.filters.author.Guáitara Martínez, Sandro DanielSubject: search.filters.subject.ANDAMIOS BIOLÓGICOS

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    Elaboración de andamios biológicos a partir de biopolímeros naturales mediante métodos manuales y bioimpresión 3D para aplicaciones en ingeniería de tejidos
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2022-09) Guáitara Martínez, Sandro Daniel; Núñez Villacís, Lorena de los Ángeles
    There are thousands of patients with injuries that cannot be adequately treated, making tissue engineering a great alternative. Although it is not limited only to this because it can also be suitable for the development of drugs and the study of diseases. The aim of this research is to elaborate different scaffolds with potential applications in tissue engineering, using natural polymers. For this purpose, freeze-drying as a manual method and 3D bioprinting were used. The solutions for the scaffolds were elaborated by dissolving chitosan and collagen in acetic acid and mixing them with an agarose solution in PBS; these solutions were freeze-dried to obtain dry and porous scaffolds. For the 3D bioprinter, the solutions were printed in the form of 4 cm square squares, with a mesh inside. They were evaluated by SEM in order to measure the pore size and analyze their surface area. Using the ethanol infiltration method, a better understanding of the pores was obtained. Water absorption capacity tests were performed. Due to the importance of biodegradability, it was evaluated by immersion in blood plasma for 2, 4 and 7 days. Finally, the biomineralization of the scaffolds was also evaluated in the same way as biodegradability, i.e., incubating in blood plasma. With the evaluation of the characteristics, it was analyzed for which tissues the scaffolds could be useful, which was mainly bone and dermal tissue.