Ciencia e Ingeniería en Alimentos y Biotecnología
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Item Posible papel estrogénico de metales pesados en cultivos de células de cáncer de mama MCF-7(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-07) Cabrera Riofrío, David Alejandro; De Lucca Osorio, MariselEstrogens could act as an important risk factor in the development of breast cancer, an increase in estrogen produced in our body, or an overexposure to environmental estrogens such as heavy metals cause a signal cascade that leads to the overactivation of oncogenic genes (cfos, c-myc) through estrogen receptors (ERs). This mechanism promotes the development of malignant phenotype, by altering the cell cycle leading to the formation of tumors. The main objective of the present project was to evaluate the estrogenic role of heavy metals in cultures of MCF-7 breast cancer cells to establish an experimental model that allows us to study the alterations that occur at the cellular level when exposing these cells to different metal concentrations. The effect of different concentrations of heavy metals (chromium, lead, mercury, nickel, cadmium and arsenic) on MCF-7 cells was analyzed by its action on cell proliferation and estrogen receptor gene expression alpha (RE-α). In addition, the binding of these metals to this receptor was examined by competition studies in the presence of estradiol-FITC, through fluorescence microscopy techniques. The results indicate that these heavy metals have an estrogenic effect on MCF-7 cells, and this effect exerts it through their binding to RE-α