Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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Tema: search.filters.subject.FERMENTACIÓN ESTÁTICA

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  • Miniatura
    Item type:Ítem,
    Evaluación del efecto del ácido tereftálico en la producción de celulosa bacteriana por Komagataeibacter xylinus DSM2004
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Alimentos, 2026-07)
    Herrera Bermudes, Erick Ricardo
    ;
    Cerda Mejía, Liliana Alexandra
    The industrial-scale production of bacterial cellulose (BC) faces limitations associated with low yields and the high costs of conventional substrates, which justify the exploration of alternative additives such as terephthalic acid (TPA), derived from PET recycling. This study evaluated the effect of TPA supplementation on BC production using the strain Komagataeibacter xylinus DSM2004. The study analyzed the interaction of three critical factors: pH, TPA concentration, and cultivation type. The results indicated that the optimal condition was pH 6.5 with 1 percent TPA under static cultivation, achieving a yield of 4.62 g per L. Supplementation with 1 percent TPA enhanced yield compared to the conventional medium; however, increasing the concentration to 2 percent resulted in an inhibitory effect, particularly under agitated conditions, where glucose consumption ceased prematurely. FTIR characterization showed that TPA did not produce detectable alterations in the functional group’s characteristic of bacterial cellulose. Mechanical analysis revealed that the presence of the monomer influenced the flexibility and stiffness of the material, suggesting a possible plasticizing effect. Overall, the results suggest that the valorization of TPA derived from PET recycling may constitute a technically viable strategy for the production of advanced biomaterials, integrating circular economy principles into biotechnology.
  • Miniatura
    Item type:Ítem,
    Obtención y caracterización de celulosa bacteriana producida por cepas acéticas endémicas del Ecuador
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09)
    Álvarez Morales, Juan José
    ;
    García Solís, Mario Daniel
    The research and development of new bacterial strains producing bacterial cellulose (BC) is a topic of great relevance today, especially in the context of biotechnology and industrial applications. This study focuses on native strains from Ecuador, with special attention to acetic bacteria PDC 21 and PDC 25, opening new possibilities for BC production. The methodology of this study involved the axenic culture of the strains and the observation of the formation of BC biofilm. Fermentative processes were carried out and the BC production yield was evaluated in comparison with a control of Komagataeibacter xylinus (DSMZ 2004). In addition, acid, thermal, and mechanical treatments were performed to obtain nanocellulose. The morphology of the samples was analyzed by scanning electron microscopy (SEM), while Fourier Transform Infrared Spectroscopy (FTIR-IR) was used to evaluate their chemical properties. The study concludes with the proposal that strain PDC 25 could be a viable alternative for BC production in industrial applications. This conclusion is based on its production yield, glucose consumption, and the properties of the BC it produces, which are similar to those of strain DSMZ 2004, commonly used as a study model and in the industry. This finding opens new possibilities for BC production and biotechnology in Ecuador.